Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis

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Molinero-Fernández, Águeda and Moreno Guzmán, María and López, Miguel Ángel and Escarpa, Alberto (2020) Magnetic Bead-Based Electrochemical Immunoassays On-Drop and On-Chip for Procalcitonin Determination: Disposable Tools for Clinical Sepsis Diagnosis. Biosensors, 10 (6). p. 66. ISSN 2079-6374

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Official URL: https://doi.org/10.3390/bios10060066




Abstract

Procalcitonin (PCT) is a known protein biomarker clinically used for the early stages of sepsis diagnosis and therapy guidance. For its reliable determination, sandwich format magnetic bead-based immunoassays with two different electrochemical detection approaches are described: (i) disposable screen-printed carbon electrodes (SPE-C, on-drop detection); (ii) electro-kinetically driven microfluidic chips with integrated Au electrodes (EMC-Au, on-chip detection). Both approaches exhibited enough sensitivity (limit of detection (LOD) of 0.1 and 0.04 ng mL−1 for SPE-C and EMC-Au, respectively; cutoff 0.5 ng mL−1), an adequate working range for the clinically relevant concentrations (0.5–1000 and 0.1–20 ng mL−1 for SPE-C and EMC-Au, respectively), and good precision (RSD < 9%), using low sample volumes (25 µL) with total assay times less than 20 min. The suitability of both approaches was successfully demonstrated by the analysis of human serum and plasma samples, for which good recoveries were obtained (89–120%). Furthermore, the EMC-Au approach enabled the easy automation of the process, constituting a reliable alternative diagnostic tool for on-site/bed-site clinical analysis.


Item Type:Article
Uncontrolled Keywords:electrochemical immunoassays; microfluidic chips; screen-printed; sepsis
Subjects:Medical sciences > Pharmacy > Pharmaceutical chemistry
Medical sciences > Pharmacy > Physical chemistry
ID Code:67557
Deposited On:30 Aug 2021 07:59
Last Modified:06 Sep 2021 09:15

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