Response of RAW 264.7 and J774A.1 macrophages to particles and nanoparticles of a mesoporous bioactive glass: A comparative study

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Feito Castellano, María José and Casarrubios Molina, Laura and Oñaderra Sánchez, Mercedes and Gómez Duro, M. and Arribas, P. and Polo Montalvo, A. and Vallet Regí, María and Arcos Navarrete, Daniel and Portolés Pérez, María Teresa (2021) Response of RAW 264.7 and J774A.1 macrophages to particles and nanoparticles of a mesoporous bioactive glass: A comparative study. Colloids and Surfaces B: Biointerfaces . ISSN 0927-7765 (In Press)

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Official URL: https://doi.org/10.1016/j.colsurfb.2021.112110




Abstract

Mesoporous bioactive glasses (MBGs) are bioceramics designed to induce bone tissue regeneration and very useful materials with the ability to act as drug delivery systems. MBGs can be implanted in contact with bone tissue in different ways, as particulate material, in 3D scaffolds or as nanospheres. In this work, we assessed the effects of particles of mesoporous bioactive glass MBG-75S and mesoporous nanospheres NanoMBG-75S on RAW 264.7 and J774A.1 macrophages, which present different sensitivity and are considered as ideal models for the study of innate immune response. After evaluating several cellular parameters (morphology, size, complexity, proliferation, cell cycle and intracellular content of reactive oxygen species), the action MBG-75S particles and NanoMBG-75S on the polarization of these macrophages towards the pro-inflammatory (M1) or reparative (M2) phenotype was determined by the expression of specific M1 (CD80) and M2 (CD206, CD163) markers. We previously measured the adsorption of albumin and fibrinogen on MBG-75S particles and the production of pro-inflammatory cytokines as TNF-α and IL-6 by macrophages in response to these particles. This comparative study demonstrates that particles of mesoporous bioactive glass MBG-75S and mesoporous nanospheres NanoMBG-75S allow the appropriated development and function of RAW 264.7 and J774A.1 macrophages and do not induce polarization towards the M1 pro-inflammatory phenotype. Therefore, considering that these mesoporous biomaterials offer the possibility of loading drugs into their pores, the results obtained indicate their high potential for use as drug-delivery systems in bone repair and osteoporosis treatments without triggering an adverse inflammatory response.


Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)
ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)
RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)
ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)

Uncontrolled Keywords:mesoporous bioactive glasses, nanomaterials, macrophages, innate immune response, biocompatibility, cytokine
Subjects:Sciences > Chemistry > Materials
ID Code:67927
Deposited On:23 Sep 2021 08:05
Last Modified:24 Sep 2021 07:23

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