Modulation of aqueous humor melatonin levels by yellow-filter and its protective effect on lens



Downloads per month over past year

LLedó Mayans, Victoria Eugenia and Alkozi, Hanan Awad and Sánchez Naves, Juan and Fernández Torres, Miguel Ángel and Guzmán Aránguez, Ana Isabel (2021) Modulation of aqueous humor melatonin levels by yellow-filter and its protective effect on lens. Journal of Photochemistry and Photobiology B: Biology, 221 (112248). ISSN 1011-1344

[thumbnail of 1-s2.0-S1011134421001275-main.pdf] PDF
Restringido a Repository staff only


Official URL:


Melatonin is mainly secreted by the pineal gland, and it is also produced by various ocular structures such as the lens. It has been recently demonstrated that melatonin ocular synthesis can be induced by blocking the blue component of white light by means of filters. Melatonin exhibits antioxidant properties that can be useful to face light-induced oxidative stress as well as oxidative events associated to ocular pathologies like cataracts. Moreover, as oxidative stress is a main event in cataract development, changes in melatonin levels could happen and be relevant in the progression of this pathology, a subject that remains uncertain. The goal of this work was to analyze the ability of a short wavelength light blocking (yellow) filter to modulate endogenous melatonin concentration and the antioxidant and cytoprotective actions induced by yellow filter’s use in lens. Furthermore, we evaluated the potential changes in aqueous humor melatonin concentration from patients with cataracts. In human lens epitheli cells, white light-emitting diode (LED) light challenge reduced melatonin secretion, protein levels of the enzymes involved in melatonin synthesis (hydroxyindole-O-methyltransferase and unphosphorylated and phosphorylated forms of arylalkylamine N-acetyltransferase) and cell viability whereas increased reactive oxygen species production. Yellow filter exposure precluded melatonin secretion reduction and protected cells from oxidative damage. Consistent with cataract patient’s results, significantly lower levels of melatonin were observed in aqueous humor of alloxan-induced diabetic cataract rabbits as compared to those of control rabbits. In contrast, aqueous humor melatonin levels of diabetic cataract animals maintaining in cages covered with a yellow filter resembled control values. This recovery seems to be mediated by the induction of melatonin biosynthetic enzymes protein expression. Yellow filter also preserved Nrf2 lens protein expression and superoxide dismutase protein levels and activity in diabetic animals. Modulation of endogenous ocular melatonin concentration using blocking filters might be a promising approach to prevent premature lens opacification.

Item Type:Article
Additional Information:

Received: 7 March 2021 / Received in revised form: 10 June 2021 / Accepted: 20 June 2021 / Available online: 23 June 2021.
In memoriam of the prominent scientist Jesus Pintor, pioneering of ocular melatonin’s research in our laboratory.

Uncontrolled Keywords:Melatonin; Cataract; Diabetes; White light-emitting diode light; Filter
Subjects:Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Visual perception
ID Code:67937
Deposited On:20 Dec 2021 14:40
Last Modified:20 Dec 2021 14:57

Origin of downloads

Repository Staff Only: item control page