Opportunistic feeding behaviour and Leishmania infantum detection in Phlebotomus perniciosus females collected in the human leishmaniasis focus of Madrid, Spain (2012–2018)

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González Fernández, Estela and Molina, Ricardo and Iriso, Andrés and Ruiz, Sonia and Aldea, Irene and Tello Fierro, Ana and Fernández Marchán, Daniel and Jiménez, Maribel (2021) Opportunistic feeding behaviour and Leishmania infantum detection in Phlebotomus perniciosus females collected in the human leishmaniasis focus of Madrid, Spain (2012–2018). PLoS Neglected Tropical Diseases, 15 (3). pp. 1-12. ISSN 1935-2727; Electronic: 1935-2735

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Official URL: https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0009240



Abstract

Background: An outbreak of human leishmaniasis due to Leishmania infantum has been registered in an urban area of southwestern Madrid, Spain, since 2010. Entomological surveys carried out in the municipalities of Fuenlabrada, Leganés, Getafe and Humanes de Madrid showed that Phlebotomus perniciosus is the only potential vector. In this work, an intensive molecular surveillance was performed in P. perniciosus females captured in the region between 2012 and 2018.
Methodology/Principal findings: A total of 1805 P. perniciosus females were analyzed for Leishmania infection, and 1189 of them also for bloodmeal identification. Eleven different species of vertebrate were detected by amplification and subsequent sequencing of the 359 bp cytb fragment. The most prevalent blood source identified was hare (n = 553, 46.51%), followed by rabbit (n = 262, 21.95%). Less frequent were cat (n = 45, 3.80%), human (n = 34, 2.90%), pig (n = 14, 1.20%), horse (n = 11, 0.93%), sheep (n = 3, 0.25%), rhea (n = 3, 0.25%), partridge (n = 1, 0.09%) and chicken (n = 1, 0.09%). The distribution of the blood meal sources varied between the different locations. Regarding L. infantum detection, PCR amplification of a fragment of kDNA, cpb gene and ITS1 region showed 162 positive specimens (8.97%). The highest infection rate was found in the municipality of Leganés (15.17%).
Conclusions: The results of this molecular survey in P. perniciosus, the only leishmaniasis vector in the outbreak occurred in southwestern Madrid region, showed its opportunistic blood-feeding behaviour, high infection rates and the differences between the different points. This study was an essential part of the intensive surveillance plan in the area and the results obtained have supported the implementation of control measures in the outbreak.


Item Type:Article
Uncontrolled Keywords:Leishmaniasis; Phlebotomus perniciosus; Madrid (Spain)
Subjects:Medical sciences > Medicine > Communicable diseases
Medical sciences > Biology > Insects
ID Code:68233
Deposited On:14 Oct 2021 12:23
Last Modified:14 Oct 2021 12:28

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