Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage



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Moura de Gouveia, Neire and Ramos, Sonia and Martín, María Ángeles and Espindola, Foued Salmen and Goya, Luis and Palomino Ruiz-Poveda, Olga María (2017) Vochysia rufa Stem Bark Extract Protects Endothelial Cells against High Glucose Damage. Medicines, 4 (1). pp. 9-11. ISSN 2305-6320

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Official URL: https://doi.org/10.3390/medicines4010009


Background: Increased oxidative stress by persistent hyperglycemia is a widely accepted factor in vascular damage responsible for type 2 diabetes complications. The plant Vochysia rufa (Vr) has been used in folk medicine in Brazil for the treatment of diabetes. Thus; the protective effect of a Vr stem bark extract against a challenge by a high glucose concentration on EA.hy926 (EA) endothelial cells is evaluated.
Methods: Vegetal material is extracted with distilled water by maceration and evaporated until dryness under vacuum. Then; it is isolated by capillary electrophoresis–tandem mass spectrometry. Cell viability is evaluated on EA cells treated with 0.5–100 µg/mL of the Vr extract for 24 h. The extract is diluted at concentrations of 5, 10 and 25 µg/mL and maintained for 24 h along with 30 mM of glucose to evaluate its protective effect on reduced glutathione (GSH); glutathione peroxidase (GPx) and reductase (GR) and protein carbonyl groups.
Results: V. rufa stem bark is composed mainly of sugars; such as inositol; galactose; glucose; mannose; sacarose; arabinose and ribose. Treatment with Vr up to 100 µg/mL for 24 h did not affect cell viability. Treatment of EA cells with 30 mM of glucose for 24 h significantly increased the cell damage. EA cells treated with 30 mM of glucose showed a decrease of GSH concentration and increased Radical Oxygen Species (ROS) and activity of antioxidant enzymes and protein carbonyl levels; compared to control. Co-treatment of EA with 30 mM glucose plus 1–10 µg/mL Vr significantly reduced cell damage while 5–25 µg/mL Vr evoked a significant protection against the glucose insult; recovering ROS; GSH; antioxidant enzymes and carbonyls to baseline levels.
Conclusion: V. rufa extract protects endothelial cells against oxidative damage by modulating ROS; GSH concentration; antioxidant enzyme activity and protein carbonyl levels.

Item Type:Article
Uncontrolled Keywords:Vochysia rufa; Sapotaceae; endothelial cells; oxidative damage; plant antioxidants
Subjects:Medical sciences > Pharmacy > Pharmacology
Medical sciences > Pharmacy > Pharmaceutical technology
ID Code:68817
Deposited On:23 Nov 2021 13:08
Last Modified:23 Nov 2021 13:23

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