Phylogenetic analysis and growth profiles of Fusarium incarnatum-equiseti species complex strains isolated from Tunisian cereals

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Jedidi, Ines and Jurado, Miguel and Cruz, Alejandra and Mounir, Mohamed Trabelsi and Said, Salem and González Jaén, María Teresa (2021) Phylogenetic analysis and growth profiles of Fusarium incarnatum-equiseti species complex strains isolated from Tunisian cereals. International Journal of Food Microbiology, 353 (10929). pp. 1-10. ISSN 0168-1605

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Official URL: https://doi.org/10.1016/j.ijfoodmicro.2021.109297



Abstract

The Fusarium incarnatum-equiseti species complex (FIESC) is a phylogenetically rich complex. It includes more than 30 cryptic phylogenetic species, making morphological identification problematic. FIESC has previously been detected in Tunisian cereals, but knowledge on the phylogeny and the ecophysiology of their species is lacking. In this work a phylogenetic analysis was performed using partial sequences of the translation elongation factor 1a gene (EF1a) of three FIESC strains isolated from barley and wheat from Tunisia, situated south in the Mediterranean basin, and additional strains from other countries. The results indicated that all Tunisian strains clustered with FIESC 5 group (F. clavum) together with other Spanish FIESC 5 strains also isolated from cereals. Growth rate profiles of the Tunisian strains were also determined on wheat and sorghum based media at a range of temperatures (15, 20, 25, 30, 35 and 40 ◦C) and water potential values (− 0.7, − 2.8, − 7.0, and − 9.8 MPa, corresponding to 0.995, 0.98, 0.95 and 0.93 aw values). Optimal growth was observed at 20–30 ◦C and between − 0.7 and − 7.0 MPa on both substrates (wheat and sorghum). The highest growth rate for the three strains was seen at 25 ◦C combined with − 2.8 MPa. The comparison between the growth profiles of Tunisian and Spanish FIESC 5 strains showed similar trends with some interesting differences regarding temperature and water potential factors. Tunisian strains seem to perform better between 15 and 30 ◦C and, notably, at even lower water potentials included − 9.8 Mpa. This might suggest that tolerance to low water potentials might be for Tunisian strains a more important selective clue than to higher temperatures. These results appeared to be consistent with a population well adapted to the present climatic conditions and predicted scenarios for North Africa.


Item Type:Article
Uncontrolled Keywords:FIESC; Phylogenetics; EF1a; Growth rate; Environmental factors; Cereal based media
Subjects:Medical sciences > Biology > Botany
Medical sciences > Biology > Genetics
Medical sciences > Biology > Microbiology
ID Code:69368
Deposited On:22 Dec 2021 12:50
Last Modified:23 Dec 2021 08:03

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