Incorporation of Superparamagnetic Iron Oxide Nanoparticles into Collagen Formulation for 3D Electrospun Scaffolds



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Estévez Amado, Manuel and Montalbano, Giorgia and Gallo Cordova, Álvaro and Ovejero, Jesús G. and Izquierdo Barba, Isabel and González Ortiz, Blanca and Tomasina, Clarissa and Moroni, Lorenzo and Vallet Regí, María and Vitale-Brovarone, Chiara and Fiorilli, Sonia (2022) Incorporation of Superparamagnetic Iron Oxide Nanoparticles into Collagen Formulation for 3D Electrospun Scaffolds. Nanomaterials, 12 . p. 181. ISSN 2079-4991

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Nowadays, there is an ever-increasing interest in the development of systems able to guide and influence cell activities for bone regeneration. In this context, we have explored for the first time the combination of type-I collagen and superparamagnetic iron oxide nanoparticles (SPIONs) to design magnetic and biocompatible electrospun scaffolds. For this purpose, SPIONs with a size of 12 nm were obtained by thermal decomposition and transferred to an aqueous medium via ligand exchange with dimercaptosuccinic acid (DMSA). The SPIONs were subsequently incorporated into type-I collagen solutions to prove the processability of the resulting hybrid formulation by means of electrospinning. The optimized method led to the fabrication of nanostructured scaffolds composed of randomly oriented collagen fibers ranging between 100 and 200 nm, where SPIONs resulted distributed and embedded into the collagen fibers. The SPIONs-containing electrospun structures proved to preserve the magnetic properties of the nanoparticles alone, making these matrices excellent candidates to explore the magnetic stimuli for biomedical applications. Furthermore, the biological assessment of these collagen scaffolds confirmed high viability, adhesion, and proliferation of both preosteoblastic MC3T3-E1 cells and human bone marrow-derived mesenchymal stem cells (hBM-MSCs).

Item Type:Article
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RESEARCHER ID M-9921-2014 (Isabel Izquierdo Barba)
ORCID 0000-0002-4139-4646 (Isabel Izquierdo Barba)
RESEARCHER ID K-4773-2015 (Blanca González Ortiz)
ORCID 0000-0002-0493-6071 (Blanca González Ortiz)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:SPIONs; type-I collagen; magnetic scaffolds; electrospinning; human bone marrowderived mesenchymal stem cells; bone regeneration; bone tissue engineering
Subjects:Sciences > Chemistry > Materials
ID Code:69457
Deposited On:10 Jan 2022 09:33
Last Modified:04 Apr 2022 14:38

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