Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction

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Gutiérrez Sarmiento, Wilbert and Sáyago Ayerdi, Sonia Guadalupe and Goñi Cambrodón, Isabel and Gutiérrez Miceli, Federico Antonio and Abud-Archila, Miguel and Rejón Orantes, José del Carmen and Rincón Rosales, Reiner and Peña Ocaña, Betsy Anaid and Ruíz Valdiviezo, Víctor Manuel (2020) Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction. Nutrients, 12 (3). p. 683. ISSN 2072-6643

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Official URL: https://doi.org/10.3390/nu12030683




Abstract

Mango (Mangifera indica L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal microbiota during colonic fermentation is unknown. In this study, the isolated IF of a novel ‘Ataulfo’ mango-based bar (snack) UV-C irradiated and non irradiated (UVMangoBand MangoB) were fermented. Colonic fermentation occurred in vitro under chemical-enzymatic, semi-anaerobic, batch culture and controlled pH colonic conditions. Changes in the structure of fecal microbiota were analyzed by 16s rRNA gene Illumina MiSeq sequencing. The community´s functional capabilities were determined in silico. The MangoB and UVMangoB increased the presence of Faecalibacterium, Roseburia, Eubacterium, Fusicatenibacter, Holdemanella, Catenibacterium, Phascolarctobacterium, Buttiauxella, Bifidobacterium, Collinsella, Prevotella and Bacteroides genera. The alpha indexes showed a decrease in microbial diversity after 6 h of colonic fermentation. The coordinates analysis indicated any differences between irradiated and non-irradiated bar. The metabolic prediction demonstrated that MangoB and UVMangoB increase the microbiota carbohydrate metabolism pathway. This study suggests that IF of mango-based bar induced beneficial changes on microbial ecology and metabolic pathway that could be promissory to prevention or treatment of metabolic dysbiosis. However, in vivo interventions are necessary to confirm the interactions between microbiota modulating and intestinal beneficial effects.


Item Type:Article
Uncontrolled Keywords:: “Ataulfo” mango; in vitro colonic fermentation; intestinal microbiota; 16S rRNA gene; bioinformatics
Subjects:Medical sciences > Pharmacy > Dietetics and nutrition
ID Code:69665
Deposited On:03 Feb 2022 12:37
Last Modified:03 Feb 2022 13:47

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