Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant

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Cañadas Benito, Olga and García-García, Andrea and Prieto, M. Auxiliadora and Pérez-Gil, Jesús (2021) Polyhydroxyalkanoate nanoparticles for pulmonary drug delivery: interaction with lung surfactant. Nanomaterials, 11 (6). pp. 1-17. ISSN 2079-4991

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Official URL: https://doi.org/10.3390/nano11061482




Abstract

Polyhydroxyalkanoates (PHA) are polyesters produced intracellularly by many bacterial species as energy storage materials, which are used in biomedical applications, including drug delivery systems, due to their biocompatibility and biodegradability. In this study, we evaluated the potential application of this nanomaterial as a basis of inhaled drug delivery systems. To that end, we assessed the possible interaction between PHA nanoparticles (NPs) and pulmonary surfactant using dynamic light scattering, Langmuir balances, and epifluorescence microscopy. Our results demonstrate that NPs deposited onto preformed monolayers of DPPC or DPPC/POPG bind these surfactant lipids. This interaction facilitated the translocation of the nanomaterial towards the aqueous subphase, with the subsequent loss of lipid from the interface. NPs that remained at the interface associated with liquid expanded (LE)/tilted condensed (TC) phase boundaries, decreasing the size of condensed domains and promoting the intermixing of TC and LE phases at submicroscopic scale. This provided the stability necessary for attaining high surface pressures upon compression, countering the destabilization induced by lipid loss. These effects were observed only for high NP loads, suggesting a limit for the use of these NPs in pulmonary drug delivery.


Item Type:Article
Uncontrolled Keywords:DPPC; POPG; PHA; Dynamic light scattering; Lipid monolayers; Relaxation kinetics; Epifluorescence microscopy; polyhydroxyalkanoates; PHA nanoparticles
Subjects:Medical sciences > Biology > Biochemistry
ID Code:69937
Deposited On:01 Feb 2022 11:51
Last Modified:17 Feb 2022 12:57

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