Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos

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Molina Fernández, Noemí and Rainieri, Sandra and Muñoz Olivas, Riansares and Oro Carretero, Paloma de and Sanz Landaluze, Jon (2021) Development of a method for assessing the accumulation and metabolization of antidepressant drugs in zebrafish (Danio rerio) eleutheroembryos. Analytical and Bioanalytical Chemistry, 413 (20). pp. 5169-5179. ISSN 1618-2642

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Official URL: https://doi.org/10.1007/s00216-021-03486-2



Abstract

Antidepressant drugs are widely used for the treatment of common mental or other psychiatric disorders such as depression, which affect about 121 million people worldwide. This widespread use has contributed to the input of these pharmaceuticals and their metabolites into the environment. The aim of this work was to develop an analytical method to quantify the most widely used antidepressant drugs, selective serotonin reuptake inhibitors (SSRI), and their main metabolites in the environment. For this, a new and reliable miniaturized extraction method based on dispersive SPE cleanup procedure for extraction of SSRI followed by derivatization with n-heptafluorobutyrylimidazole, and detection by GC-MS was developed. The methodology, including a firstorder one-compartment model, was then applied to a bioconcentration study in zebrafish (Danio rerio) eleutheroembryos. The results showed low bioaccumulation of these compounds; however, a biotransformation evidence of the parent compounds into their metabolites was observed after 6 h of exposure. These results indicate the need to integrate metabolic transformation rates to fully model and understand the bioaccumulation patterns of SSRI and their metabolites.


Item Type:Article
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CRUE-CSIC (Acuerdos Transformativos 2021)

Uncontrolled Keywords:Bioconcentration, SSRIs and metabolites, GC-MS, Zebrafish eleutheroembryos
Subjects:Sciences > Chemistry
Sciences > Chemistry > Analytic chemistry
ID Code:70131
Deposited On:07 Feb 2022 12:23
Last Modified:22 Feb 2022 09:58

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