Effect of gold nanostars plus amikacin against cabapenem-resistant Klebsiella pneumoniae biofilm.

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Aguilera Correa, John Jairo and García Álvarez, Rafaela and Mediero Muñoz, Aránzazu and Esteban, Jaime and Vallet Regí, María (2022) Effect of gold nanostars plus amikacin against cabapenem-resistant Klebsiella pneumoniae biofilm. Biology, 11 (2). p. 162. ISSN 2079-7737

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Official URL: https://doi.org/10.3390/biology11020162




Abstract

(1) Background: Carbapenem-resistant Klesiella pneumoniae (CR-KP) infection rates depict an almost pre-antibiotic scenario since the pipeline for effective antibiotics against this pathogen has been almost entirely depleted. This study aims to evaluate the antibacterial effect of gold nanostars (GNS) alone or associated with some of the most widely used antibiotics for the treatment of CR-KP strains, i.e., meropenem or amikacin, on both planktonic and sessile forms. Additionally, we measured the effect of GNS on cell proliferation and biocompatibility in invertebrate in vivo models.
(2) Materials and methods: GNS were made from gold seeds grown using a seeded-growth surfactant-free method assisted by silver ions and functionalized with mercapto-poly(ethylene glycol)amino by ligand exchange. The antimicrobial capacity, effect on cell proliferation, and biocompatibility of the most effective combination was evaluated in a Galleria mellonella model.
(3) Results:The minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were 80 and 160 µM of GNS for all strains, respectively. The minimum biofilm inhibitory concentration (MBIC) and minimum biofilm eradication concentration (MBEC) were >320 µM of GNS for both. A synergy was found between GNS and amikacin. Larvae administered GNS plus amikacin were found to tolerate the treatment well, which prevented infection.
(4) Conclusions: GNS are a promising anti-CR-KP nanomaterial.


Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:carbapenem-resistant K. pneumoniae; gold nanostars; amikacin; biofilm
Subjects:Sciences > Chemistry > Materials
ID Code:70152
Deposited On:07 Feb 2022 14:44
Last Modified:07 Feb 2022 14:47

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