Cell therapy for factor V deficiency: an approach based on human decidua mesenchymal stem cells

Impacto

Downloads

Downloads per month over past year

Serrano, Luis J. and De la Torre, Paz and Liras, Antonio and Flores, Ana I. (2021) Cell therapy for factor V deficiency: an approach based on human decidua mesenchymal stem cells. Biomedicine & pharmacotherapy, 142 (112059). pp. 1-8. ISSN 0753-3322

[thumbnail of Serrano L. J. 2021. Cell therapy for factor V deficiency.....pdf]
Preview
PDF
Creative Commons Attribution Non-commercial No Derivatives.

6MB

Official URL: https://doi.org/10.1016/j.biopha.2021.112059



Abstract

Deficiency of factor V is a congenital autosomal recessive coagulopathy associated with mutations in the F5 gene that results in mild-to-severe bleeding episodes. Factor V is a component of the prothrombinase complex responsible for accelerating conversion of prothrombin to thrombin. At the present time there are no therapeutic factor V concentrates available. This study was designed to lay the preliminary foundations for future cell-based therapy for patients with severe factor V deficiency. The study showed that hepatospheres, which produce coagulation factors VIII, IX, and V, synthetize and store intracellular glycogen and express albumin levels up to 8 times higher than those of undifferentiated cells. Factor IX and factor V gene expression increased significantly in hepatospheres as compared to undifferentiated cells, whereas factor VIII gene expression remained constant. The factor V protein was detected in the hepatospheres´ secretome. Considering the enormous potential of mesenchymal stem cells as therapeutic agents, this study proposes a highly reproducible method to induce differentiation of mesenchymal stem cells from human placenta to factor V-producing hepatospheres. This strategy constitutes a preliminary step towards a curative treatment of factor V deficiency through advanced therapies such as cell therapy.


Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2021)

Uncontrolled Keywords:Inherited coagulopathies; Severe human factor V; Deficiency; Cell therapy; Human decidua; Mesenchymal stem cells; Hepatocytes
Subjects:Medical sciences > Medicine > Hematology
Medical sciences > Biology > Biotechnology
ID Code:70213
Deposited On:09 Feb 2022 09:03
Last Modified:21 Feb 2022 11:18

Origin of downloads

Repository Staff Only: item control page