Enhancement of Cryo-EM maps by a multiscale tubular filter

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Vargas Balbuena, Javier and Gómez Pedrero, José Antonio and Quiroga Mellado, Juan Antonio and Alonso Fernández, José (2022) Enhancement of Cryo-EM maps by a multiscale tubular filter. Optics express, 30 (3). pp. 4515-4527. ISSN 1094-4087

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Official URL: http://dx.doi.org/10.1364/OE.444675




Abstract

We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures and to attenuate blob-like and plate-like structures. The approach, thus, introduces a priori information in the reconstructions to improve their interpretability and analysis at high-resolution. The proposed method has been tested with simulated and real cryo-EM maps including recent reconstructions of the SARS-CoV-2. Our results show that our methods can improve obtained reconstructions.


Item Type:Article
Additional Information:

© 2022 Optica Publishing Group.
Funding. Ministerio de Ciencia e Innovación (PID2019-108850RA-I00).

Uncontrolled Keywords:Resolution
Subjects:Sciences > Physics > Optics
ID Code:70705
Deposited On:11 Mar 2022 15:24
Last Modified:14 Mar 2022 10:26

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