Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases

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Torrente Rodríguez, Rebeca M. and Muñoz San Martín, Cristina and Gamella Carballo, Maria and Pedrero Muñoz, María and Martínez-Bosch, Neus and Navarro, Pilar and García de Frutos, Pablo and Pingarrón Carrazón, José Manuel and Campuzano Ruiz, Susana (2021) Electrochemical Immunosensing of ST2: A Checkpoint Target in Cancer Diseases. Biosensors, 11 (6). p. 202. ISSN 2079-6374

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Official URL: https://doi.org/10.3390/bios11060202




Abstract

A magnetic beads (MB)-involved amperometric immunosensor for the determination of ST2, a member of the IL1 receptor family, is reported in this work. The method utilizes a sandwich immunoassay and disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates built on the surface of carboxylic acid-microsized magnetic particles (HOOC-MBs) were used to selectively capture ST2. A biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP) was used to accomplish the sandwiching of the target protein. The immune platform exhibits great selectivity and a low limit of detection (39.6 pg mL−1) for ST2, allowing the determination of soluble ST2 (sST2) in plasma samples from healthy individuals and patients diagnosed with pancreatic ductal adenocarcinoma (PDAC) in only 45 min once the immunoconjugates have been prepared. The good correlation of the obtained results with those provided by an ELISA kit performed using the same immunoreagents demonstrates the potential of the developed strategy for early diagnosis and/or prognosis of the fatal PDAC disease.


Item Type:Article
Uncontrolled Keywords:electrochemical immune platform; human ST2; plasma; pancreatic cancer
Subjects:Medical sciences > Medicine > Oncology
ID Code:70833
Deposited On:28 Feb 2022 17:35
Last Modified:01 Mar 2022 09:26

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