Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis



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Zhu, Longfei and Fernández Jiménez, Nadia and Szymanska Lejmana, Maja and Pelé, Alexandre and Underwood, Charles J. and Serra, Heïdi and Lambing, Christophe and Dluzewska, Julia and Bieluszewski, Tomasz and Pradillo, Mónica and Henderson, Ian R. and Ziolkowski, Piotr A. (2021) Natural variation identifies SNI1, the SMC5/6 component, as a modifier of meiotic crossover in Arabidopsis. Proceedings of the National Academy of Sciences, 118 (33). pp. 1-12. ISSN 0027-8424, ESSN: 1091-6490

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The frequency and distribution of meiotic crossovers are tightly controlled; however, variation in this process can be observed both within and between species. Using crosses of two natural Arabidopsis thaliana accessions, Col and Ler, we mapped a crossover modifier locus to semidominant polymorphisms in SUPPRESSOR OF NPR1-1 INDUCIBLE 1 (SNI1), which encodes a component of the SMC5/6 complex. The sni1 mutant exhibits a modified pattern of recombination across the genome with crossovers elevated in chromosome distal regions but reduced in pericentromeres. Mutations in SNI1 result in reduced crossover interference and can partially restore the fertility of a Class I crossover pathway mutant, which suggests that the protein affects noninterfering crossover repair. Therefore, we tested genetic interactions between SNI1 and both RECQ4 and FANCM DNA helicases, which showed that additional Class II crossovers observed in the sni1 mutant are FANCM independent. Furthermore, genetic analysis of other SMC5/6 mutants confirms the observations of crossover redistribution made for SNI1. The study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants.

Item Type:Article
Uncontrolled Keywords:Meiosis; Crossover; SNI1; SMC5/6; Arabidopsis
Subjects:Medical sciences > Biology > Botany
Medical sciences > Biology > Genetics
ID Code:71056
Deposited On:09 Mar 2022 11:36
Last Modified:06 May 2022 10:51

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