Paving the Way for Reliable Alzheimer’s Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing

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Valverde de la Fuente, Alejandro and Gordón Pidal, José María and Montero Calle, Ana and Arévalo Pérez, Beatriz and Serafín González-Carrato, Verónica and Calero, Miguel and Moreno Guzmán, María and López, Miguel Ángel and Escarpa, Alberto and Yáñez Sedeño, Paloma and Barderas, Rodrigo and Campuzano Ruiz, Susana and Pingarrón Carrazón, José Manuel (2022) Paving the Way for Reliable Alzheimer’s Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing. ChemElectroChem . ISSN 2196-0216

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Official URL: https://doi.org/10.1002/celc.202200055



Abstract

Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values.


Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Alzheimer’s disease, Candidate biomarkers, Electrochemical, multiplexing immunoplatform, Magnetic microbeads, Plasma
Subjects:Sciences > Chemistry
Sciences > Chemistry > Analytic chemistry
Medical sciences > Medicine > Neurosciences
ID Code:71456
Deposited On:23 Mar 2022 13:02
Last Modified:05 May 2022 07:32

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