The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida albicans



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Hidalgo Vico, Susana and Prieto Prieto, Antonio Daniel and Alonso Monge, Rebeca and Román González, Elvira and Pla Alonso, Jesús (2021) The Glyoxylate Cycle Is Involved in White-Opaque Switching in Candida albicans. Journal of Fungi, 7 (7). p. 502. ISSN 2309-608X

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Candida albicans is a commensal yeast that inhabits the gastrointestinal tract of humans. The master regulator of the white-opaque transition WOR1 has been implicated in the adaptation to this commensal status. A proteomic analysis of cells overexpressing this transcription factor (WOR1OE) suggested an altered metabolism of carbon sources and a phenotypic analysis confirmed this alteration. The WOR1OE cells are deficient in using trehalose and xylose and are unable to use 2C sources, which is consistent with a reduction in the amount of Icl1, the isocitrate lyase enzyme. The icl1∆/∆ mutants overexpressing WOR1 are deficient in the production of phloxine B positive cells, a main characteristic of opaque cells, a phenotype also observed in mating type hemizygous mtla1∆ icl1∆/∆ cells, suggesting the involvement of Icl1 in the adaptation to the commensal state. In fact, icl1∆/∆ cells have reduced fitness in mouse gastrointestinal tract as compared with essentially isogenic heterozygous ICL1/icl1∆, but overproduction of WOR1 in an icl1∆/∆ mutant does not restore fitness. These results implicate the glyoxylate shunt in the adaptation to commensalism of C. albicans by mechanisms that are partially independent of WOR1.

Item Type:Article
Uncontrolled Keywords:Commensalism; Glyoxylate cycle; Wo switching; Fungal pathogenesis; Epigenetics; Opaque cells; Oxidative stress
Subjects:Medical sciences > Pharmacy > Microbiology
ID Code:71875
Deposited On:22 Apr 2022 11:34
Last Modified:25 Apr 2022 07:59

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