Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors

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Torrente Rodríguez, Rebeca Magnolia and Montero Calle, Ana and San Bartolomé, Clara and Cano, Olga and Vazquez, Monica and Iglesias Caballero, María and Corral Lugo, Andrés and McConnell, Michael J. and Pascal, Mariona and Mas, Vicente and Pingarrón Carrazón, José Manuel and Barderas, Rodrigo and Campuzano Ruiz, Susana (2022) Towards Control and Oversight of SARS-CoV-2 Diagnosis and Monitoring through Multiplexed Quantitative Electroanalytical Immune Response Biosensors. Angewandte Chemie International Edition . ISSN 1433-7851

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Official URL: https://doi.org/10.1002/anie.202203662



Abstract

The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built-on the surface of magnetic microbeads functionalized with nucleocapsid (N) and inhouse expressed recombinant spike (S) proteins is reported.
Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8) screen-printed electrodes using the HQ/HRP/H2O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency and even identification of the variant responsible for the infection.


Item Type:Article
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CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:SARS-CoV-2, humoral immune response, multiplexed quantitative electroanalytical bioplatforms, in-house expressed viral antigens, Omicron
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:72120
Deposited On:12 May 2022 11:36
Last Modified:29 Sep 2022 10:14

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