¡Nos trasladamos! E-Prints cerrará el 7 de junio.

En las próximas semanas vamos a migrar nuestro repositorio a una nueva plataforma con muchas funcionalidades nuevas. En esta migración las fechas clave del proceso son las siguientes:

Es muy importante que cualquier depósito se realice en E-Prints Complutense antes del 7 de junio. En caso de urgencia para realizar un depósito, se puede comunicar a docta@ucm.es.

Arsenate and arsenite differential toxicity in Tetrahymena thermophila



Downloads per month over past year

Rodriguez Martín, Daniel and Murciano Cespedosa, Antonio and Herráiz Moreno, Marta and Francisco Martínez, Patricia de and Amaro Torres, Francisco and Gutiérrez Fernández, Juan Carlos and Martín-González, Ana María and Díaz del Toro, Silvia (2022) Arsenate and arsenite differential toxicity in Tetrahymena thermophila. Journal of Hazardous Materials, 431 (128532). pp. 1-16. ISSN 0304-3894

[thumbnail of Rodríguez-Martín D. et al. 2002. Arsenate and arsenite differential toxicity....pdf]
Creative Commons Attribution.


Official URL: https://doi.org/10.1016/j.jhazmat.2022.128532


A comparative analysis of toxicities of both arsenic forms (arsenite and arsenate) in the model eukaryotic microorganism Tetrahymena thermophila (ciliate protozoa) has shown the presence of various detoxification mechanisms and cellular effects comparable to those of animal cells under arsenic stress. In the wild type strain SB1969 arsenate is almost 2.5 times more toxic than arsenite. According to the concentration addition model used in binary metallic mixtures their toxicities show an additive effect. Using fluorescent assays and flow cytometry, it has been detected that As(V) generates elevated levels of ROS/RNS compared to As(III). Both produce the same levels of superoxide anion, but As(V) also causes greater increases in hydrogen peroxide and peroxynitrite. The mitochondrial membrane potential is affected by both As(V) and As(III), and electron microscopy has also revealed that mitochondria are the main target of both arsenic ionic forms. Fusion/fission and swelling mitochondrial and mitophagy, together with macroautophagy, vacuolization and mucocyst extruction are mainly associated to As(V) toxicity, while As(III) induces an extensive lipid metabolism dysfunction (adipotropic effect). Quantitative RT-PCR analysis of some genes encoding antioxidant proteins or enzymes has shown that glutathione and thioredoxin metabolisms are involved in the response to arsenic stress. Likewise, the function of metallothioneins seems to be crucial in arsenic detoxification processes, after using both metallothionein knockout and knockdown strains and cells overexpressing metallothionein genes from this ciliate. The analysis of the differential toxicity of As(III) and As(V) shown in this study provides cytological and molecular tools to be used as biomarkers for each of the two arsenic ionic forms.

Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Tetrahymena; Arsenite and arsenate; Toxicity; Mitochondrial dysfunction; Metallothioneins; Antioxidant gene expression
Subjects:Medical sciences > Medicine > Toxicology
Medical sciences > Biology > Invertebrates
Medical sciences > Biology > Microbiology
ID Code:72493
Deposited On:26 May 2022 09:23
Last Modified:26 May 2022 10:10

Origin of downloads

Repository Staff Only: item control page