Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples



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Ortega Becerril, José Antonio and Infantes Lorenzo, José Antonio and Roy, A. and Juan Ferré, Lucía de and Romero Martínez, Beatriz and Moreno, I. and Domínguez, M. and Domínguez Rodríguez, Lucas and Bezos Garrido, Javier (2022) Factors affecting the performance of P22 ELISA for the diagnosis of caprine tuberculosis in milk samples. Research in Veterinary Science, 145 . pp. 40-45. ISSN 00345288

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Official URL: https://doi.org/10.1016/j.rvsc.2022.02.008


Caprine tuberculosis (TB) is a zoonosis caused by members of the Mycobacterium tuberculosis complex (MTBC). Caprine TB eradication programmes are based mainly on intradermal tuberculin tests and slaughterhouse surveillance. However, the use of serological test has been extended as a potential diagnostic tool in goats through the use of serum, plasma, or even milk samples. Milk production and the antibodies (Ab) present in milk can vary depending on several circumstances. In the present study, different factors that may affect the performance of humoral TB diagnosis were analysed using goat milk samples: 1) lactation stage, 2) a recent previous skin test (booster effect) and 3) the effect of freeze-thaw cycles on milk samples preserved with azidiol. TB-infected animals (n = 44) were selected to evaluate the evolution of the Ab levels during the 6-month lactation period, along with its potential effect on the P22 ELISA results. In general, no significant changes (p = 0.079) were observed throughout the study as regards Ab levels in milk samples between consecutive analysis although the reactivity to P22 ELISA decreased when samplings were performed at the last two months of the lactation. Regarding the booster effect, the quantitative results showed a significant variation (p < 0.001) for both milk and serum samples when serological tests were carried out 15 days after the skin test. Finally, there were no significant differences (p = 0.99) in the P22 ELISA results when using milk samples preserved with azidiol that had undergone freeze-thaw cycles.

Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Caprine tuberculosis, Milk, P22 ELISA, Lactation, Azidiol
Subjects:Medical sciences > Veterinary > Sheep
Medical sciences > Veterinary > Veterinary pathology
ID Code:72605
Deposited On:30 May 2022 14:32
Last Modified:30 May 2022 15:22

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