Xerotolerance: a new property in Exiguobacterium Genus

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Castillo López, María and Galán, Beatriz and Carmona, Manuel and Navarro LLorens, Juana María and Peretó, Juli and Porcar, Manuel and Getino, Luis and Olivera, Elías R. and Luengo, José M. and Castro, Laura and García, José Luis (2021) Xerotolerance: a new property in Exiguobacterium Genus. Microorganisms, 9 (12). pp. 1-20. ISSN Electronic: 2076-2607

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Official URL: https://doi.org/10.3390/microorganisms9122455




Abstract

The highly xerotolerant bacterium classified as Exiguobacterium sp. Helios isolated from a solar panel in Spain showed a close relationship to Exiguobacterium sibiricum 255-15 isolated from Siberian permafrost. Xerotolerance has not been previously described as a characteristic of the extremely diverse Exiguobacterium genus, but both strains Helios and 255-15 showed higher xerotolerance than that described in the reference xerotolerant model strain Deinococcus radiodurans. Significant changes observed in the cell morphology after their desiccation suggests that the structure of cellular surface plays an important role in xerotolerance. Apart from its remarkable resistance to desiccation, Exiguobacterium sp. Helios strain shows several polyextremophilic characteristics that make it a promising chassis for biotechnological applications. Exiguobacterium sp. Helios cells produce nanoparticles of selenium in the presence of selenite linked to its resistance mechanism. Using the Lactobacillus plasmid pRCR12 that harbors a cherry marker, we have developed a transformation protocol for Exiguobacterium sp. Helios strain, being the first time that a bacterium of Exiguobacterium genus has been genetically modified. The comparison of Exiguobacterium sp. Helios and E. sibiricum 255-15 genomes revealed several interesting similarities and differences. Both strains contain a complete set of competence-related DNA transformation genes, suggesting that they might have natural competence, and an incomplete set of genes involved in sporulation; moreover, these strains not produce spores, suggesting that these genes might be involved in xerotolerance.


Item Type:Article
Uncontrolled Keywords:Exiguobacterium; Xerotolerance; Biotechnology; Polyextremophile; Desiccation resistance
Subjects:Medical sciences > Biology > Molecular biology
Medical sciences > Biology > Biochemistry
Medical sciences > Biology > Microbiology
ID Code:72635
Deposited On:01 Jun 2022 10:29
Last Modified:14 Jun 2022 10:57

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