Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm

Impacto

Downloads

Downloads per month over past year

Aldea, Irene and Gibello Prieto, Alicia and Hernández, Marta and Leekitcharoenphon, Pimlapas and Bortolaia, Valeria and Moreno Romo, Miguel Ángel (2022) Clonal and plasmid-mediated flow of ESBL/AmpC genes in Escherichia coli in a commercial laying hen farm. Veterinary Microbiology, 270 . p. 109453. ISSN 03781135

[thumbnail of 1-s2.0-S0378113522001237-main.pdf]
Preview
PDF
Creative Commons Attribution No Derivatives.

1MB

Official URL: https://doi.org/10.1016/j.vetmic.2022.109453



Abstract

Resistance to third- and fourth-generation cephalosporins in Escherichia coli is mainly due to extended-spectrum beta-lactamases (ESBL) and AmpC cephalosporinases, which have been increasingly reported, mainly in isolates from humans and poultry. The aim of this study was to address the flow of antimicrobial resistance determinants in the full laying hen production cycle (four batches followed from day-old chicks to 83/84-week-old layers), using cephalosporinresistant E. coli as a model and their characterization using whole genome sequencing (WGS). Fifteen out of 22 samples analysed yielded growth on MacConkey agar with cefotaxime (1 mg/L). Of these, 141 isolates were identified as E. coli and 47 were characterized by WGS. Genes detected were three ESBL (blaCTX-M-1 (n = 19); blaCTX-M-14 (n = 1); and blaSHV-12 (n = 9)) and one AmpC (blaCMY-2 (n = 13)). Some isolates only harboured blaTEM-1B (n = 2) or blaTEM-1D (n = 1). IncI1 plasmids were the main platform for ESBL/AmpC genes. In addition, five clones were identified harbouring blaCTX-M-1 (two), blaSHV-12 (one) and blaCMY-2 (two), drawing a clone-plasmid mixed flow model. Gene blaCTX-M-1 was found in the chromosomal DNA of clone 1 over 14 months, and in IncI1/ST3 plasmids over six months; over six months blaSHV-12 was found harboured by clone 3 (IncI1/ST26 plasmids), and 15 months later in a non-replicon detected plasmid. Finally, blaCMY-2 spread for at least 16 months, mainly by IncK2 (including clone 4) and IncI1/ST12 (clone 5) plasmids. Proper use of antimicrobials should be combined with other farm management strategies for the effective control of cephalosporin-resistant E. coli isolates in commercial layer farms.


Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:CTX-M-1, SHV-12, CMY-2, Egg production, Dynamics, Cephalosporin resistance
Subjects:Medical sciences > Veterinary
Medical sciences > Veterinary > Aviculture
Medical sciences > Veterinary > Animal culture
ID Code:72644
Deposited On:01 Jun 2022 10:20
Last Modified:02 Jun 2022 06:54

Origin of downloads

Repository Staff Only: item control page