Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA

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Aedo, Jorge and Aravena Canales, Daniela and Ruiz Jarabo, Ignacio and Oyarzún, Ricardo and Molina, Alfredo and Martínez Rodríguez, Gonzalo and Valdés, Juan Antonio and Mancera, Juan Miguel (2021) Differential Metabolic and Transcriptional Responses of Gilthead Seabream (Sparus aurata) Administered with Cortisol or Cortisol-BSA. Animals, 11 (11). pp. 1-15. ISSN Electronic: 2076-2615

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Official URL: https://doi.org/10.3390/ani11113310




Abstract

Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, Sparus aurata were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in S. aurata enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in S. aurata.


Item Type:Article
Uncontrolled Keywords:Cortisol; Energetic metabolism; Fish; Glucocorticoids; Sparus aurata; Stress response
Subjects:Medical sciences > Biology > Animal physiology
Medical sciences > Biology > Fishes
ID Code:72731
Deposited On:07 Jun 2022 10:51
Last Modified:14 Jun 2022 10:47

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