Rapid and Reproducible MALDI-TOF-Based Method for the Detection of Vancomycin-Resistant Enterococcus faecium Using Classifying Algorithms

Impacto

Downloads

Downloads per month over past year

Candela, Ana and Arroyo, Manuel J. and Sánchez Molleda, Ángela and Méndez, Gema and Quiroga, Lidia and Ruiz, Adrián and Cercenado Mansilla, Emilia and Maron Arriaza, Mercedes and Muñoz, Patricia and Mancera, Luis and Rodríguez Temporal, David and Rodríguez Sánchez, Belén (2022) Rapid and Reproducible MALDI-TOF-Based Method for the Detection of Vancomycin-Resistant Enterococcus faecium Using Classifying Algorithms. Diagnostics, 12 (2). p. 328. ISSN 2075-4418

[thumbnail of diagnostics-12-00328-v2.pdf]
Preview
PDF
Creative Commons Attribution.

3MB

Official URL: https://doi.org/10.3390/diagnostics12020328




Abstract

Vancomycin-resistant Enterococcus faecium represents a health threat due to its ability to spread and cause outbreaks. MALDI-TOF MS has demonstrated its usefulness for E. faecium identification, but its implementation for antimicrobial resistance detection is still under evaluation. This study assesses the repeatability of MALDI-TOF MS for peak analysis and its performance in the discrimination of vancomycin-susceptible (VSE) from vancomycin-resistant isolates (VRE). The study was carried out on protein spectra from 178 E. faecium unique clinical isolates—92 VSE, 31 VanA VRE, 55 VanB VRE-, processed with Clover MS Data Analysis software. Technical and biological repeatability were assayed. Unsupervised (principal component analysis, (PCA)) and supervised algorithms (support vector machine (SVM), random forest (RF) and partial least squares–discriminant analysis (PLS-DA)) were applied. The repeatability assay was performed with 18 peaks common to VSE and VRE with intensities above 1.0% of the maximum peak intensity. It showed lower variability for normalized data and for the peaks within the 3000–9000 m/z range. It was found that 80.9%, 79.2% and 77.5% VSE vs. VRE discrimination was achieved by applying SVM, RF and PLS-DA, respectively. Correct internal differentiation of VanA from VanB VRE isolates was obtained by SVM in 86.6% cases. The implementation of MALDI-TOF MS and peak analysis could represent a rapid and effective tool for VRE screening. However, further improvements are needed to increase the accuracy of this approach.


Item Type:Article
Uncontrolled Keywords:enterococci; vancomycin resistance; MALDI-TOF; mass spectrometry; peak analysis
Subjects:Medical sciences > Medicine > Communicable diseases
ID Code:72854
Deposited On:29 Jun 2022 14:39
Last Modified:30 Jun 2022 07:36

Origin of downloads

Repository Staff Only: item control page