Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study



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Fernández Albarral, José Antonio and Hoz Montañana, María Rosa de and Matamoros, José A. and Chen, Leijing and López Cuenca, Inés and García Martín, Elena Salobrar and Sánchez Puebla, Lídia and Ramirez Sebastian, Jose Manuel and Triviño Casado, Alberto and Salazar Corral, Juan José and Ramírez Sebastián, Ana Isabel (2022) Retinal Changes in Astrocytes and Müller Glia in a Mouse Model of Laser-Induced Glaucoma: A Time-Course Study. Biomedicines, 10 (5). 30 p.. ISSN 2227-9059

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Macroglia (astrocytes and Müller glia) may play an important role in the pathogenesis of glaucoma. In a glaucoma mouse model, we studied the effects of unilateral laser-induced ocular hypertension (OHT) on macroglia in OHT and contralateral eyes at different time points after laser treatment (1, 3, 5, 8 and 15 days) using anti-GFAP and anti-MHC-II, analyzing the morphological changes, GFAP-labelled retinal area (GFAP-PA), and GFAP and MHC-II immunoreactivity intensities ((GFAP-IRI and MHC-II-IRI)). In OHT and contralateral eyes, with respect to naïve eyes, at all the time points, we found the following: (i) astrocytes with thicker somas and more secondary processes, mainly in the intermediate (IR) and peripheral retina (PR); (ii) astrocytes with low GFAP-IRI and only primary processes near the optic disc (OD); (iii) an increase in total GFAP-RA, which was higher at 3 and 5 days, except for at 15 days; (iv) an increase in GFAP-IRI in the IR and especially in the PR; (v) a decrease in GFAP-IRI near the OD, especially at 1 and 5 days; (vi) a significant increase in MHC-II-IRI, which was higher in the IR and PR; and (vii) the Müller glia were GFAP+ and MHC-II+. In conclusion, in this model of glaucoma, there is a bilateral macroglial activation maintained over time involved in the inflammatory glaucoma process.

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Received: 17 March 2022 / Revised: 15 April 2022 / Accepted: 16 April 2022 / Published: 19 April 2022
This article belongs to the Special Issue The Contribution of Astrocytes to Human Neurodegenerative Diseases.

Uncontrolled Keywords:experimental glaucoma; mice; microglia; astrocytes; Müller cell; retina; GFAP; MHC-II; ocular hypertension; contralateral eye
Subjects:Medical sciences > Medicine > Ophtalmology
Medical sciences > Optics > Eyes anatomy
ID Code:73144
Deposited On:27 Jun 2022 12:21
Last Modified:28 Jun 2022 11:02

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