Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS

Impacto

Downloads

Downloads per month over past year

Romero Sánchez, Iván and Ramírez García, Lorena and Gracia Lor, Emma and Madrid Albarrán, Yolanda (2022) Simultaneous Determination of Aflatoxins B1, B2, G1 and G2 IN Commercial Rices Using Immunoaffinity Column Clean-UP and HPLC-MS/MS. Food Chemistry, 395 . p. 133611. ISSN 0308-8146

[thumbnail of 1-s2.0-S0308814622015734-main.pdf]
Preview
PDF
Creative Commons Attribution Non-commercial No Derivatives.

797kB

Official URL: https://doi.org/10.1016/j.foodchem.2022.133611



Abstract

Rice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable results were obtained by using methanol – water (80:20, v/v) extraction followed by immunoaffinity columns for clean-up, with recoveries of 86–92%, standard deviations between 5 and 11%, LOD ranged between 0.09 and 0.32 µg/kg, and LOQ between 0.31 and 1.06 µg/kg. Method validation and sample analysis were performed by using HPLC-MS/MS. Nine rice samples from different origin, varieties and specific characteristics, acquired in Spanish supermarkets were analysed. In two basmati samples from the same batch aflatoxin B1 was detected at (1.62 ± 0.08) µg/kg and (0.77 ± 0.03) µg/kg, both lower than the levels established by European Regulation for aflatoxin B1 in cereals.


Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Aflatoxins, Cereals, Rice, Immunoaffinity columns, HPLC-MS/MS, Bioanalytical Chemistry
Subjects:Sciences > Chemistry > Analytic chemistry
ID Code:73457
Deposited On:04 Aug 2022 07:52
Last Modified:04 Aug 2022 08:54

Origin of downloads

Repository Staff Only: item control page