Identifcation of novel extracellular putative chitinase and hydrolase from Geomyces sp. B10I with the biodegradation activity towards polyesters

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Urbanek, Aneta K. and Arroyo Sánchez, Miguel and Mata Riesco, Isabel de la and Mirończuk, Aleksandra M. (2022) Identifcation of novel extracellular putative chitinase and hydrolase from Geomyces sp. B10I with the biodegradation activity towards polyesters. AMB Express, 12 (12). pp. 1-11. ISSN Electronic: 2191-0855

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Official URL: https://doi.org/10.1186/s13568-022-01352-7




Abstract

Cold-adapted flamentous fungal strain Geomyces sp. B10I has been reported to decompose polyesters such as poly(e-caprolactone) (PCL), poly(butylene succinate) (PBS) and poly(butylene succinate-co-butylene adipate) (PBSA). Here, we identifed the enzymes of Geomyces sp. B10I, which appear to be responsible for its biodegradation activity. We compared their amino acid sequences with sequences of well-studied fungal enzymes. Partial purifcation of an extracellular mixture of the two enzymes, named hydrGB10I and chitGB10I, using ammonium sulfate precipitation and ionic exchange chromatography gave 14.16-fold purity. The amino acid sequence of the proteins obtained from the MALDI-TOF analysis determined the molecular mass of 77.2 kDa and 46.5 kDa, respectively. Conserved domain homology analysis revealed that both proteins belong to the class of hydrolases; hydrGB10I belongs to the glycosyl hydrolase 81 superfamily, while chitGB10I contains the domain of the glycosyl hydrolase 18 superfamily. Phylogenetic analysis suggests a distinct nature of the hydrGB10I and chitGB10I of Geomyces sp. B10I when compared with other fungal polyester-degrading enzymes described to date.


Item Type:Article
Uncontrolled Keywords:Geomyces sp. B10I; Polyesters; Chitinase; Hydrolase; Cold-adapted microorganisms
Subjects:Medical sciences > Biology > Biochemistry
Medical sciences > Biology > Microbiology
ID Code:73930
Deposited On:19 Jul 2022 08:55
Last Modified:03 Aug 2022 07:17

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