Sugar concentration, nitrogen availability, and phylogenetic factors determine the ability of Acinetobacter spp. and Rosenbergiella spp. to grow in floral nectar

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Morales Poole, José R. and Vega, Clara de and Tsuji, Kaoru and Jacquemyn, Hans and Junker, Robert R. and Herrera, Carlos M. and Michiels, Chris and Lievens, Bart and Álvarez Pérez, Sergio (2022) Sugar concentration, nitrogen availability, and phylogenetic factors determine the ability of Acinetobacter spp. and Rosenbergiella spp. to grow in floral nectar. Microbial Ecology . ISSN 1432-184X

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Official URL: https://doi.org/10.1007/s00248-022-02088-4



Abstract

The floral nectar of angiosperms harbors a variety of microorganisms that depend predominantly on animal visitors for their dispersal. Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar. We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose). Growth was only observed in four of the 12 artificial nectars. Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth. Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny. Overall, these results demonstrate that the ability of Acinetobacter spp. and Rosenbergiella spp. to grow in floral nectar largely depends on nectar chemistry and bacterial phylogeny.


Item Type:Article
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CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Acinetobacter, floral nectar, bee, phylogeny, Rosenbergiella
Subjects:Medical sciences > Biology > Ecology
Medical sciences > Biology > Microbiology
ID Code:74113
Deposited On:09 Aug 2022 07:31
Last Modified:09 Aug 2022 07:31

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