Publication: Caracterización de la formación de P-bodies y de la función de la proteína Pat1 en situaciones de estrés que afectan a la integridad celular de Saccharomyces cerevisiae
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2022-08-12
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Universidad Complutense de Madrid
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Abstract
La pared fúngica es una estructura dinámica y esencial cuya función es proteger a la célula frente a condiciones medioambientales adversas, garantizando la viabilidad celular. La adaptación de la levadura a situaciones que producen alteraciones sobre la pared celular está regulada principalmente por la ruta de MAPKs de integridad celular (CWI), vía la MAPK Slt2, la cual se activa por fosforilación bajo estas condiciones. Esta activación induce cambios en el programa transcripcional de la célula con el fin de adaptarse al estrés. En múltiples situaciones de estrés, las células eucariotas, incluidas las levaduras, acumulan agregados citoplasmáticos formados por mRNA y proteínas, como los Processing-bodies (PBs). Los PBs se han asociado tanto con degradación de mRNA, ya que en su proteoma abundan elementos pertenecientes a la maquinaria de degradación de mRNA, como con su almacenaje para su posterior traducción. De hecho, se ha descrito el tránsito de mRNAs entre polisomas y PBs, lo que sugiere su participación en la regulación de la expresión génica a través del control de la traducción de mRNAs. Los PBs también se han relacionado con distintas enfermedades humanas poniendo de relevancia su importancia biológica...
The fungal cell wall is a dynamic and essential structure whose function is to protect the cell against adverse environmental conditions, ensuring cell viability and survival. The adaptation of yeast to situations that can produce damage to the cell wall is mainly regulated by MAPK Cell Wall Integrity (CWI) pathway, via the MAPK Slt2, which is activated by phosphorylation under these conditions. This activation induces changes in the transcriptional program of the cell to adapt it to stress and survive under adverse conditions. In multiple stress situations, eukaryotic cells, including yeasts, accumulate cytoplasmic aggregates consisting of mRNA and proteins, such as Processing-bodies (PBs). PBs have been associated with mRNA degradation due to elements belonging to the mRNA degradation machinery being abundant in their proteome, and withm RNAs storage sites for subsequent translation. The movement of mRNAs between polysomes and PBs has been described, suggesting their involvement in the regulation of gene expression through the control of mRNA translation. PBs have also been linked to several human diseases, highlighting their biological importance...
The fungal cell wall is a dynamic and essential structure whose function is to protect the cell against adverse environmental conditions, ensuring cell viability and survival. The adaptation of yeast to situations that can produce damage to the cell wall is mainly regulated by MAPK Cell Wall Integrity (CWI) pathway, via the MAPK Slt2, which is activated by phosphorylation under these conditions. This activation induces changes in the transcriptional program of the cell to adapt it to stress and survive under adverse conditions. In multiple stress situations, eukaryotic cells, including yeasts, accumulate cytoplasmic aggregates consisting of mRNA and proteins, such as Processing-bodies (PBs). PBs have been associated with mRNA degradation due to elements belonging to the mRNA degradation machinery being abundant in their proteome, and withm RNAs storage sites for subsequent translation. The movement of mRNAs between polysomes and PBs has been described, suggesting their involvement in the regulation of gene expression through the control of mRNA translation. PBs have also been linked to several human diseases, highlighting their biological importance...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Farmacia, leída el 09-06-2022