Publication: Nuevos avances en el estudio de la interacción de PPRV con el sistema inmune y evaluación de un candidato vacunal DIVA
Loading...
Official URL
Full text at PDC
Publication Date
2022-10-06
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
El virus de la peste de los pequeños rumiantes (PPRV) es un morbillivirus, familia Paramyxoviridae, estrechamente relacionado con los virus del sarampión y la peste bovina. Es el agente causal de la peste de los pequeños rumiantes (PPR), una enfermedad de grave impacto económico que afecta en especial a los países en vías de desarrollo con gran dependencia del ganado ovino y caprino. Las células dendríticas (DC) son células presentadoras de antígeno profesionales, que actúan como nexo entre la inmunidad innata y la inmunidad adaptativa. Son responsables del desarrollo de una correcta respuesta inmune frente a los patógenos y, a la vez, diana del virus. Nuestro objetivo fue evaluar si la infección con PPRV podía alterar la funcionalidad de las DC, obstaculizando el desarrollo de una respuesta inmune adaptativa eficaz frente al virus. Las DC pueden encontrarse principalmente en dos estados funcionales generales, inmaduro y maduro, por lo que se estudió el efecto de la infección por PPRV en ambas etapas. Las células CD14+ (monocitos) seleccionadas positivamente a partir de células mononucleares de sangre periférica (PBMC) se diferenciaron a DC derivadas de monocito inmaduras (iMoDC) con GM-CSF e IL-4. Las iMoDC se maduraron a DC derivadas de monocito maduras (mMoDC) mediante la transfección de Poly I:C. La infección con PPRV modificó la expresión de marcadores clave en las DC, tales como MHC-I, MHC-II, CD80,CD86, CD14, CD209, CD11b y CD11c, tanto en iMoDC como en mMoDC. La funcionalidad de las MoDC también se vio alterada por PPRV: la capacidad fagocítica de las iMoDC infectadas estaba reducida, mientras que las mMoDC infectadas eran incapaces de estimular linfocitos T...
Peste des Petits Ruminants virus (PPRV) is a Morbillivirus, Paramyxoviridae family, closely related to measles and rinderpest virus. It is the causative agent of Peste des Petits Ruminants (PPR), an economically important disease, which severely impacts developing countries with heavy dependence on sheep and goat livestock. Dendritic cells (DC), which are professional antigen presenting cells that act as a bridge between the innate and the adaptive immunity to trigger a correct immune response to pathogens, are among the immune cells targeted by the virus. Our goal was to assess whether PPRV infection could alter DC functionality, hindering the development of an efficient adaptive immune response against the virus. Since DC primarily exist in two basic functional states, immature and mature, we studied the effects of PPRV infection in both cell phases. CD14+ cells (monocytes) positively selected from Peripheral Blood Mononuclear Cells (PBMC) were differentiated into immature monocyte-derived DC (iMoDC) with GM-CSF and IL-4. iMoDC were matured into mature monocyte-derived DC (mMoDC) by Poly I:C transfection. Expression of key DC markers MHCI, MHC-II, CD80, CD86, CD14, CD209, CD11b and CD11c was affected by PPRV infection in iMoDC and mMoDC. PPRV infection also altered MoDC functionality: phagocytic activity of infected iMoDC was reduced, while infected mMoDC were unable to stimulate T cells...
Peste des Petits Ruminants virus (PPRV) is a Morbillivirus, Paramyxoviridae family, closely related to measles and rinderpest virus. It is the causative agent of Peste des Petits Ruminants (PPR), an economically important disease, which severely impacts developing countries with heavy dependence on sheep and goat livestock. Dendritic cells (DC), which are professional antigen presenting cells that act as a bridge between the innate and the adaptive immunity to trigger a correct immune response to pathogens, are among the immune cells targeted by the virus. Our goal was to assess whether PPRV infection could alter DC functionality, hindering the development of an efficient adaptive immune response against the virus. Since DC primarily exist in two basic functional states, immature and mature, we studied the effects of PPRV infection in both cell phases. CD14+ cells (monocytes) positively selected from Peripheral Blood Mononuclear Cells (PBMC) were differentiated into immature monocyte-derived DC (iMoDC) with GM-CSF and IL-4. iMoDC were matured into mature monocyte-derived DC (mMoDC) by Poly I:C transfection. Expression of key DC markers MHCI, MHC-II, CD80, CD86, CD14, CD209, CD11b and CD11c was affected by PPRV infection in iMoDC and mMoDC. PPRV infection also altered MoDC functionality: phagocytic activity of infected iMoDC was reduced, while infected mMoDC were unable to stimulate T cells...
Description
Tesis inédita de la Universidad Complutense de Madrid, Facultad de Veterinaria, leída el 07-04-2022