Cu-doped hollow bioactive glass nanoparticles for bone infec-tion treatment



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Jiménez Holguín, Javier and Sánchez Salcedo, Sandra and Cicuendez, Monica and Vallet Regí, María and Salinas Sánchez, Antonio J. (2022) Cu-doped hollow bioactive glass nanoparticles for bone infec-tion treatment. Pharmaceutics, 14 . p. 845. ISSN 1999-4923

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In search of new approaches to treat bone infection and prevent drug resistance development, a nanosystem based on hollow bioactive glass nanoparticles (HBGN) of composition 79.5SiO2–(18-x)CaO–2.5P2O5–xCuO (x = 0, 2.5 or 5 mol-% CuO) was developed. The objective of the study was to evaluate the capacity of the HBGN to be used as nanocarriers of the broad-spectrum anti-biotic danofloxacin and source of bactericidal Cu2+ ions. Core-shell nanoparticles with specific surface areas close to 800 m2/g and pore volumes around 1 cm3/g were obtained by using hexa-decyltrimethylammonium bromide (CTAB) and poly (styrene)-block-poly (acrylic acid) (PS-b-PAA) as structure-directing agents. Flow cytometry studies showed the cytocompatibility of the nanoparticles in MC3T3-E1 pre-osteoblastic cell cultures. Ion release studies confirmed the release of non-cytotoxic concentrations of Cu2+ ions within the therapeutic range. Moreover, it was shown that the inclusion of copper in the system resulted in a more gradual release of da-nofloxacin that was extended over one week. The bactericidal activity of the nanosystem was evaluated with E. coli and S. aureus strains. Nanoparticles with copper were not able to reduce bacterial viability by themselves and Cu-free HBGN failed to reduce bacterial growth, despite releasing higher antibiotic concentrations. However, HBGN enriched with copper and da-nofloxacin drastically reduced bacterial growth in sessile, planktonic and biofilm states, which was attributed to a synergistic effect between the action of Cu2+ ions and danofloxacin. There-fore, the nanosystem here investigated is a promising candidate as an alternative for the local treatment of bone infections.

Item Type:Article
Additional Information:

RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)
RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez)
ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)

Uncontrolled Keywords:Hollow nanoparticles; Mesoporous glasses; Copper; Antibacterial infection pre-osteoblasts
Subjects:Sciences > Chemistry > Materials
Medical sciences > Pharmacy > Inorganic chemistry
ID Code:75488
Deposited On:14 Nov 2022 08:37
Last Modified:14 Nov 2022 16:42

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