In Vitro and In Vivo Response of Zinc-Containing Mesoporous Bioactive Glasses in a Sheep Animal Model



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Jiménez Holguín, Javier and Arcos Navarrete, Daniel and Lozano Borregón, Daniel and Sainz-Pardo Sanz, Melchor and Ortega Medina, Luis and Enciso, S and Fernández Tomé, Blanca and López Güemes, Idoia and Sánchez Margallo, Francisco Miguel and Casarrubios Molina, Laura and Portolés Pérez, María Teresa and Vallet Regí, María (2022) In Vitro and In Vivo Response of Zinc-Containing Mesoporous Bioactive Glasses in a Sheep Animal Model. International Journal of Molecular Sciences, 23 (22). p. 13918. ISSN 1422-0067

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Zinc-enriched mesoporous bioactive glasses (MBGs) are bioceramics with potential antibacterial and osteogenic properties. However, few assays have been performed to study these properties in animal models.In this study, MBGs enriched with up to 5% ZnO were synthesized, physicochemically characterized, and evaluated for their osteogenic activity both in vitro and in vivo.
The ZnO MBGs showed excellent textural properties despite ZnO incorporation. However, the release of Zn2+ ions inhibited the mineralization process when immersed in simulated body fluid. In vitro assays showed significantly highe r values of viability and expression of early markers of celldifferentiation and angiogenesis in a ZnO-content-dependent manner. The next step was to study the osteogenic potential in a sheep bone defect model. Despite their excellent textural properties and cellular response in vitro, the ZnO MBGs were not able to integrate into the bone tissue, which can be explained in terms of inhibition of the mineralization process caused by Zn2+ ions. This work highlights the need to develop nanostructured materials for bone regeneration that can mineralize to interact with bone tissue and induce the processes of implant acceptance, cell colonization by osteogenic cells, and regeneration of lost bone tissue.

Item Type:Article
Additional Information:

RESEARCH ID B-5081-2017  (Daniel Lozano Borregón)
ORCID 0000-0001-5902-9201 (Daniel Lozano Borregón)
RESEARCHER ID M-3378-2014 (María Vallet Regí)
ORCID 0000-0002-6104-4889 (María Vallet Regí)

Uncontrolled Keywords:mesoporous bioactive glass; zinc; bone regeneration; bioactivity; cell cultures; in vivo study
Subjects:Sciences > Chemistry > Materials
ID Code:75590
Deposited On:17 Nov 2022 08:57
Last Modified:17 Nov 2022 10:02

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