Biosensing Tacrolimus in Human Whole Blood by Using a Drug Receptor Fused to the Emerald Green Fluorescent Protein



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Glahn Martínez, Bettina and Lucchesi, Giacomo and Pradanas González, Fernando and Manzano, Ana Isabel and Canales Mayordomo, Ángeles and Caminati, Gabriella and Benito Peña, Elena and Moreno Bondi, María C. (2022) Biosensing Tacrolimus in Human Whole Blood by Using a Drug Receptor Fused to the Emerald Green Fluorescent Protein. Analytical Chemistry . ISSN 0003-2700

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Tacrolimus (FK506) is an immunosuppressant drug (ISD) used to prevent organ rejection after transplantation that exhibits a narrow therapeutic window and is subject to wide inter- and intra-individual pharmacokinetic fluctuations requiring careful monitoring. The immunosuppressive capacity of FK506 arises from the formation of a complex with immunophilin FKBP1A. This paper describes the use of FKBP1A as an alternative to common antibodies for biosensing purposes. Bioassays use recombinant FKBP1A fused to the emerald green fluorescent protein (FKBP1A–EmGFP). Samples containing the immunosuppressant are incubated with the recombinant protein, and free FKBP1A–EmGFP is captured by magnetic beads functionalized with FK506 to generate a fluorescence signal. Recombinant receptor–drug interaction is evaluated by using a quartz crystal microbalance and nuclear magnetic resonance. The limit of detection (3 ng mL–1) and dynamic range thus obtained (5–70 ng mL–1) fulfill therapeutic requirements. The assay is selective for other ISD usually coadministered with FK506 and allows the drug to be determined in human whole blood samples from organ transplant patients with results comparing favorably with those of an external laboratory.

Item Type:Article
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CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Anatomy, Assays, Fluorescence, Genetics, Peptides, proteins
Subjects:Sciences > Chemistry > Analytic chemistry
Sciences > Chemistry > Chemistry, Organic
ID Code:75702
Deposited On:22 Nov 2022 13:02
Last Modified:22 Nov 2022 13:12

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