Study of the functional relationships between the buccinator muscle and the connective tissue of the cheek in humans



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Martínez Sanz, Elena and Catón Vázquez, Javier and Maldonado Bautista, Estela and Murillo González, Jorge Alfonso and Barrio Asensio, María Carmen and Paradas Lara, Irene and García Serradilla, Moisés and Arráez Aybar, Luis and Mérida Velasco, José Ramón (2022) Study of the functional relationships between the buccinator muscle and the connective tissue of the cheek in humans. Annals of Anatomy, 246 . p. 152025. ISSN 0940-9602, ESSN: 1618-0402

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Background: The buccinator muscle derives from the mesenchyme of the second pharyngeal arch. In adults, it has a quadrilateral shape, occupying the deepest part of the cheek region. Its function is complex, being active during swallowing, chewing, and sucking. To our knowledge, there are no studies that have specifically analyzed the relationship of the buccinator muscle fibers and neighboring connective tissue of the cheek in humans, neither during development nor in adults. Such relationships are fundamental to understand its function. Thus, in this study the relations of the buccinator muscle with associated connective tissue were investigated.
Methods: The buccinator muscle region was investigated bilaterally in 41 human specimens of 8–17 weeks of development. Moreover, four complete adult tissue blocks from human cadavers (including mucosa and skin) were obtained from the cheek region (between the anterior border of the masseter muscle and the nasolabial fold). All samples were processed with standard histological techniques. In addition, subsets of sections were stained with picrosirius red (PSR). Furthermore, immunoreactivity against type I and III collagen was also studied in adult tissues.
Results: The buccinator muscle showed direct relationships with its connective tissue from 8 to 17 weeks of development. Collagen fibers were arranged in septa from the submucosa to the skin through the muscle. These septa were positive for type I collagen and presented elastic fibers. Fibrous septa that were positive for type III collagen were arranged from the lateral side of the muscle to the skin.
Conclusions: The intimate relationship between buccinator muscle fibers and cheek connective tissue may explain the complex functions of this muscle.

Item Type:Article
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CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Buccinator muscle, Connective tissue, Functional anatomy, Human, Collagens type I/III, Immunohistochemistry, Picrosirius red staining, Polarized light microscope
Subjects:Medical sciences > Medicine > Anatomy
ID Code:76054
Deposited On:20 Dec 2022 16:04
Last Modified:20 Dec 2022 16:05

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