First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen

Impacto

Downloads

Downloads per month over past year

Gamella, Maria and Laza, Anabel and Parrón Ballesteros, Jorge and Bueno, Cristina and Ruiz Valdepeñas Montiel, Víctor and Pedrero, María and Bertolino, Franco A. and Pingarrón, José M. and Villalba, Mayte and Campuzano, Susana (2023) First PCR-free electrochemical bioplatform for the detection of mustard Sin a 1 protein as a potential “hidden” food allergen. Bioelectrochemistry, 150 . p. 108357. ISSN 15675394

[thumbnail of 1-s2.0-S1567539422003085-main.pdf]
Preview
PDF
Creative Commons Attribution Non-commercial No Derivatives.

1MB

Official URL: https://doi.org/10.1016/j.bioelechem.2022.108357




Abstract

A disposable electrochemical PCR-free biosensor for the selective detection of a fragment encoding the protein Sin a 1, a 2S albumin considered a diagnostic marker for sensitization to mustard, is reported. The methodology is based on the formation of DNA/RNA heterohybrids by sandwich hybridization of a specific fragment of the Sin a 1 allergen coding sequence with appropriately designed RNA probes. Labeling with commercial antibodies specific to the heteroduplexes and secondary antibodies conjugated with horseradish peroxidase (HRP) was carried out onto the surface of magnetic beads (MBs). Amperometric transduction was undertaken on screen-printed electrodes using H2O2 as enzyme substrate and hydroquinone (HQ) a redox mediator. The electrochemical biosensor allows the simple and fast detection (75 min) of Sin a 1 reaching a limit of detection of 3 pM. The bioplatform was successfully applied to the analysis of the targeted Sin a 1 gene specific region using just 50 ng of non-fragmented denatured genomic DNA extracted from yellow mustard seeds.


Item Type:Article
Additional Information:

CRUE-CSIC (Acuerdos Transformativos 2022)

Uncontrolled Keywords:Electrochemical bioplatform; PCR-free; Mustard; Sin a 1 gene; Food allergen
Subjects:Medical sciences > Biology > Biochemistry
ID Code:76736
Deposited On:15 Mar 2023 12:23
Last Modified:15 Mar 2023 12:23

Origin of downloads

Repository Staff Only: item control page