The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces

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Alonso Español, Andrea and Bravo Fernández, Enrique and Ribeiro Vidal, Honorato José and Virto Ruiz, Leire and Herrera González, David and Alonso Álvarez, Bettina María and Sanz Alonso, Mariano (2023) The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces. International Journal of Molecular Sciences, 24 (3). art.2335. ISSN 1422-0067

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Official URL: https://doi.org/10.3390/ijms24032335




Abstract

In search for natural products with antimicrobial properties for use in the prevention and treatment of peri-implantitis, the purpose of this investigation was to evaluate the antimicrobial activity of curcumin and xanthohumol, using an in vitro multi-species dynamic biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of curcumin (5 mM) and xanthohumol (100 μM) extracts, and the respective controls, were evaluated with 72-h biofilms formed over dental implants by their submersion for 60 seconds. The evaluation was assessed by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). For the data analysis, comparisons were tested applying ANOVA tests with post-hoc Bonferroni corrections to evaluate the antimicrobial activity of both extracts. With qPCR, statistically significant reductions in bacterial counts were observed for curcumin and xanthohumol, when compared to the negative control. The results with CLSM and SEM were consistent with those reported with qPCR. It was concluded that both curcumin and xanthohumol have demonstrated antimicrobial activity against the six bacterial species included in the dynamic in vitro biofilm model used.


Item Type:Article
Uncontrolled Keywords:Curcumin; Xanthohumol; Peri-implantitis; Oral biofilms; Scanning electron microscopy; Confocal laser microscopy; Polymerase chain reaction; In vitro; Antibacterial; Antibiofilm
Subjects:Medical sciences > Medicine
Medical sciences > Medicine > Dentistry
Medical sciences > Medicine > Optics
ID Code:77311
Deposited On:28 Apr 2023 08:27
Last Modified:28 Apr 2023 08:32

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