Publication: Molecular characterization of lizard parasites and their influence on colour ornaments
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2016-03-01
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Universidad Complutense de Madrid
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Abstract
La presente tesis doctoral ha abordado dos objetivos principales: 1) la caracterización morfológica y molecular de los protozoos eimeriorinos más prevalentes en lagartos (Schellackia, Lankesterella, Acroeimeria, Choleoeimeria, Caryospora e Isospora), así como su análisis filogenético y 2) el efecto que ejercen sobre la expresión de los ornamentos de color en diferentes sistemas parásito-hospedador. Para llevar a cabo el primer objetivo se tomaron muestras de heces y tejido sanguíneo de lagartos silvestres y también de lagartos presentes en tiendas especializadas. Las muestras sanguíneas fueron obtenidas mediante la punción de la base de la cola con una aguja estéril. La sangre extraída se utilizó para realizar un diagnóstico directo, mediante frotis sanguíneo, y uno molecular a partir de las muestras conservadas en tarjetas FTA-Whatman. Las muestras para el estudio de los parásitos intestinales se obtuvieron a partir de heces recogidas de la cloaca de los hospedadores. Tras la homogeneización de la muestra, una parte se utilizó para realizar el diagnóstico coprológico mientras que otra parte fue empleada para la identificación molecular de los parásitos detectados mediante la amplificación del gen nuclear 18S ribosomal. Una vez caracterizados molecularmente los coccidios hemáticos o intestinales, se procedió a la realización de un análisis filogenético para esclarecer las relaciones evolutivas existentes entre estos géneros, desconocidos en gran medida, de coccidios...
This dissertation achieved two main objectives: 1) the morphologic, molecular and phylogenetic characterization of the most prevalent eimeriorine protozoan found in reptiles (Schellackia, Lankesterella, Acroeimeria, Choleoeimeria, Caryospora and Isospora) and, 2) the effect of the parasitosis produced by some of these pathogens over the conspicuousness of coloured ornaments in different lizard host-parasite systems. For the first objective, we got blood and fecal samples from both free-ranging and captive lizards from pet stores. We obtained the blood samples from the coccygeal vein with a steril needle. This blood was used for a direct diagnostic by both microscopy and molecular screening (we targeted the 18S rRNA gene). Similarly, the screening of the fecal samples was used for diagnosing intestinal coccidiosis by both microscopy and molecular amplification of 18S rRNA gene. After the molecular characterization of hematic and intestinal coccidia, we included the sequences from these parasites in Bayesian and Maxima likelyhood phylogenetic trees to understand the evolutionary affinities among these genera of coccidia...
This dissertation achieved two main objectives: 1) the morphologic, molecular and phylogenetic characterization of the most prevalent eimeriorine protozoan found in reptiles (Schellackia, Lankesterella, Acroeimeria, Choleoeimeria, Caryospora and Isospora) and, 2) the effect of the parasitosis produced by some of these pathogens over the conspicuousness of coloured ornaments in different lizard host-parasite systems. For the first objective, we got blood and fecal samples from both free-ranging and captive lizards from pet stores. We obtained the blood samples from the coccygeal vein with a steril needle. This blood was used for a direct diagnostic by both microscopy and molecular screening (we targeted the 18S rRNA gene). Similarly, the screening of the fecal samples was used for diagnosing intestinal coccidiosis by both microscopy and molecular amplification of 18S rRNA gene. After the molecular characterization of hematic and intestinal coccidia, we included the sequences from these parasites in Bayesian and Maxima likelyhood phylogenetic trees to understand the evolutionary affinities among these genera of coccidia...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Ciencias Biológicas, Departamento de Zoología y Antropología Física, leída el 13-01-2016