Publication: Efectos de la administración exógena de rhGH y de la ingesta de dietas con lactoalbúmina sobre el perfil lipídico, la actividad paraoxonasa y parámetros ponderales en ratones BALB/c adultos y viejos
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2016-08-10
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Universidad Complutense de Madrid
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Abstract
El progreso de la edad lleva en los seres vivos al envejecimiento como un proceso ineludible y viene acompañado de una serie de alteraciones y procesos deletéreos que no son exclusivos de la vejez. En este estudio se seleccionaron dos estrategias antienvejecimiento: por un lado la estrategia endocrina consistente en la administración exógena de hormona de crecimiento humana recombinante (rhGH), y por otro la estrategia nutricional que propone el suministro de una proteína de alta calidad nutricional como es la lactoalbúmina en dos concentraciones en la dieta. Los objetivos de esta tesis doctoral fueron los siguientes: 1)Aplicar dos estrategias antienvejecimiento: el tratamiento con rhGH y la alimentación con dietas conteniendo dos concentraciones de lactoalbúmina (14% y 20%) a ratones BALB/c maduros y viejos, para determinar sus efectos, así como la diferencia de edad, sobre los parámetros ponderales, la ingesta, y la distribución de sustratos corporales, en especial la grasa y la proteína, así como la razón proteína/grasa. 2)Estudiar, los efectos de dichas estrategias y la edad, sobre el perfil lipídico y la actividad de la enzima paraoxonasa (PON1). 3)Evaluar los posibles efectos de los factores (EDAD, TRATAMIENTO y DIETA), así como de las interacciones de dichos factores (análisis de la varianza) y el estudio posterior de las diferencias de entre grupos (comparación post hoc). 4)Realizar estudios comparativos con controles a dos niveles. Por una parte, con controles del tratamiento endocrino y nutricional, comparando ratones tratados con rhGH frente a salinos, así como 20% frente a 14%; y en segundo lugar comparando los ratones alimentados con las dietas experimentales frente a controles alimentados con una dieta comercial control. 5)Se realizó un estudio histológico del parénquima hepático para poder comprobar y comparar el estado de los hepatocitos entre los diversos grupos experimentales y controles...
Aging in animals is accompanied by the apparition of deleterious processes, not always exclusive of old age. In the search for strategies struggling aging and its unwanted effects, this study tested two strategies antiaging and their interactions: the exogenous treatment with recombinant human Growth Hormone (rhGH) on one hand, and the nutrition with a high quality protein source as lactalbumin on the other hand. The objectives of this study were: 1)To apply two anti-aging strategies, namely: rhGH treatment and feeding two dietary lactalbumin levels (14% or 20%), to adult and old BALB/c mice to study its effects, as well as the age effect, on feed intake, corporal parameters, body composition, specially body protein, body fat and the relationship between them. 2)To study the effects of the two strategies and the age effect on the lipid profile and the paraoxonase (PON1) activity. 3)To Evaluate the possible effects of the factors studied (AGE, TREATMENT and DIET), as well as the interactions among the factors by means of the ANOVA analysis and post hoc multiple comparison analysis. 4)Controls were used at two levels: on the first level, controls were of two types depending on the factor considered i.e. rhGH vs. saline, and 20% diet vs. 14% diet. Whereas the second level of controls were used to compare the experimental lactalbumin diets vs. a maintenance commercial diet. 5)Finally, a histological study of the liver was performed to test the hepatocyte status among the different groups of mice...
Aging in animals is accompanied by the apparition of deleterious processes, not always exclusive of old age. In the search for strategies struggling aging and its unwanted effects, this study tested two strategies antiaging and their interactions: the exogenous treatment with recombinant human Growth Hormone (rhGH) on one hand, and the nutrition with a high quality protein source as lactalbumin on the other hand. The objectives of this study were: 1)To apply two anti-aging strategies, namely: rhGH treatment and feeding two dietary lactalbumin levels (14% or 20%), to adult and old BALB/c mice to study its effects, as well as the age effect, on feed intake, corporal parameters, body composition, specially body protein, body fat and the relationship between them. 2)To study the effects of the two strategies and the age effect on the lipid profile and the paraoxonase (PON1) activity. 3)To Evaluate the possible effects of the factors studied (AGE, TREATMENT and DIET), as well as the interactions among the factors by means of the ANOVA analysis and post hoc multiple comparison analysis. 4)Controls were used at two levels: on the first level, controls were of two types depending on the factor considered i.e. rhGH vs. saline, and 20% diet vs. 14% diet. Whereas the second level of controls were used to compare the experimental lactalbumin diets vs. a maintenance commercial diet. 5)Finally, a histological study of the liver was performed to test the hepatocyte status among the different groups of mice...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Farmacia, Sección Departamental de Fisiología (Fisiología Animal), leída el 28-01-2016