Publication: Caracterización de la proteína DiiA de "Streptococcus pneumoniae" y evaluación de su potencial como antígeno vacunal
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2018-02-21
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Universidad Complutense de Madrid
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Abstract
Streptococcus pneumoniae (neumococo) puede encontrarse en el hombre formando parte de la microbiota de la nasofaringe aunque también puede diseminarse produciendo enfermedades invasivas y no invasivas, principalmente en niños, ancianos y pacientes inmunodeprimidos (Henriques-Normark y Tuomanen, 2013). Sin embargo, muchos aspectos de la patobiología de este microorganismo no se conocen en profundidad, concretamente la transición de comensal a patógeno. Aunque el principal factor de virulencia de neumococo es su cápsula polisacarídica (McDaniel y Swiatlo, 2004), las proteínas de superficie también tienen un papel fundamental en la patogénesis de la bacteria. Estas proteínas interaccionan directamente con el hospedador permitiendo, por ejemplo, la colonización de la nasofaringe, la internalización a través del epitelio pulmonar y la invasión de otros tejidos por parte de la bacteria (Kadioglu et al., 2008). Las proteínas de superficie que median la adhesión presentan típicamente una arquitectura que incluye un dominio de anclaje a la pared celular, un tallo desestructurado que se proyecta hacia el exterior y en el extremo más distal – secuencias repetidas que suelen plegarse como láminas beta (Lin et al., 2012). El número de repeticiones puede variar entre aislados clínicos, lo que puede condicionar tanto la afinidad por la molécula diana como la inmunogenicidad de la proteína (Gravekamp et al., 1997)...
Streptococcus pneumoniae (pneumococcus) can be found in humans taking part of the nasopharyngeal microbiota, although it can spread causing invasive and non-invasive disease, mainly in children, elderly and immunocompromised patients (Henriques-Normark and Tuomanen, 2013). However, many aspects of the pathobiology of this bacterium are not well understood, in particular the transition from commensalism to virulence. Although the major virulence factor of pneumococcus is its polysaccharide capsule (McDaniel and Swiatlo, 2004), surface proteins play a fundamental role in the pathogenesis of the bacterium. These proteins interact directly with the host mediating, for example, colonization of the nasopharynx, internalization through alveolar epithelium and invasion of other tissues (Kadioglu et al., 2008). Surface proteins, which are involved in adhesion process, present a typical architecture that includes a cell wall anchoring domain, an unstructured stalk projected to the outside and - at the furthest point - repeated sequences that usually are β-strands (Lin et al., 2012). The number of repeats varies among clinical isolates, which may affect both the affinity to the target molecule and the immunogenicity of the protein (Gravekamp et al., 1997)...
Streptococcus pneumoniae (pneumococcus) can be found in humans taking part of the nasopharyngeal microbiota, although it can spread causing invasive and non-invasive disease, mainly in children, elderly and immunocompromised patients (Henriques-Normark and Tuomanen, 2013). However, many aspects of the pathobiology of this bacterium are not well understood, in particular the transition from commensalism to virulence. Although the major virulence factor of pneumococcus is its polysaccharide capsule (McDaniel and Swiatlo, 2004), surface proteins play a fundamental role in the pathogenesis of the bacterium. These proteins interact directly with the host mediating, for example, colonization of the nasopharynx, internalization through alveolar epithelium and invasion of other tissues (Kadioglu et al., 2008). Surface proteins, which are involved in adhesion process, present a typical architecture that includes a cell wall anchoring domain, an unstructured stalk projected to the outside and - at the furthest point - repeated sequences that usually are β-strands (Lin et al., 2012). The number of repeats varies among clinical isolates, which may affect both the affinity to the target molecule and the immunogenicity of the protein (Gravekamp et al., 1997)...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Farmacia, Departamento de Microbiología II, leída el 2-06-2017