Publication: Caracterización de la proteína recombinante rHc23 y mejora de su capacidad inmunizante frente a la hemoncosis ovina
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2019-04-25
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Universidad Complutense de Madrid
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Abstract
La hemoncosis ovina es una enfermedad parasitaria de distribución mundial causada por el nematodo gástrico Haemonchus contortus. La patogenia de esta parasitosis se relaciona con la hematofagia de adultos y preadultos del helminto. La enfermedad es responsable de importantes pérdidas económicas debido a la disminución de la producción y calidad de carne, leche y lana en los animales adultos, así como la muerte de los corderos más afectados. Su control se ha basado esencialmente en el uso de antihelmínticos. No obstante, el aumento de las resistencias frente a estos fármacos,la falta de nuevos antiparasitarios y la creciente preocupación social en la agricultura sostenible, han provocado interés en el desarrollo de sistemas alternativos de control tales como la inmunoprofilaxis. Los estudios en el ganado ovino están limitados por su coste y el desconocimiento de muchos aspectos de su sistema inmunitario, por lo que el empleo de modelos subrogados de laboratorio sería relevante. Se ha logrado la vacunación de corderos frente a la hemoncosis con algunos antígenos purificados del helminto. Sin embargo, las formas recombinantes homólogas no han producido, en general, resultados comparables. La proteína recombinante rHc23, desarrollada a partir de un antígeno de adultos de H.contortus, ha sido una excepción ya que ha logrado niveles significativos de protección en corderos..
Sheep haemonchosis is a worldwide distributed parasitic disease caused by the abomasal nematode Haemonchus contortus. Pathogenesis relates to the blood feeding of preadult and adult stages of the helminth. Disease is responsible of important economic losses due to diminished production and quality of meat, milk and wool and also to the death of the most affected lambs. Control of the infection has relied almost completely on the use of anthelmintics. However, resistance phenomena, unavailability of new antiparasitic agents and the growing social concern on sustainable agriculture have fostered the interest on alternative control systems such as immunoprophylaxis. Experimental studies in sheep are hampered by their cost and the lack of information on many aspects of sheep immune system; thus the use of surrogate laboratory models would be relevant. Previous studies have shown the possibility of vaccination agains thaemonchosis with several purified antigens of the helminth. However no comparable results have been obtained, in general, with their recombinant counterparts. Recombinant protein rHc23, developed from an antigen of adult H.contortus, has been an exception since it has been able to elicit significant levels of protection in lambs...
Sheep haemonchosis is a worldwide distributed parasitic disease caused by the abomasal nematode Haemonchus contortus. Pathogenesis relates to the blood feeding of preadult and adult stages of the helminth. Disease is responsible of important economic losses due to diminished production and quality of meat, milk and wool and also to the death of the most affected lambs. Control of the infection has relied almost completely on the use of anthelmintics. However, resistance phenomena, unavailability of new antiparasitic agents and the growing social concern on sustainable agriculture have fostered the interest on alternative control systems such as immunoprophylaxis. Experimental studies in sheep are hampered by their cost and the lack of information on many aspects of sheep immune system; thus the use of surrogate laboratory models would be relevant. Previous studies have shown the possibility of vaccination agains thaemonchosis with several purified antigens of the helminth. However no comparable results have been obtained, in general, with their recombinant counterparts. Recombinant protein rHc23, developed from an antigen of adult H.contortus, has been an exception since it has been able to elicit significant levels of protection in lambs...
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Tesis de la Universidad Complutense de Madrid, Facultad de Veterinaria, Departamento de Sanidad Animal, leída el 08-11-2018