Publication: Bacteriemias de origen periodontal: validación de técnicas microbiológicas
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2020-11-06
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Universidad Complutense de Madrid
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Abstract
ANTECEDENTES: El paso de bacterias a sangre (bacteriemias) se ha postulado como uno de los posibles mecanismos responsables de la relación entre la periodontitis y ciertas enfermedades sistémicas. Pocos son los estudios que buscan bacteriemias de origen periodontal que, a su vez, analicen y correlacionen los datos obtenidos en las muestras de sangre con los obtenidos en muestras subgingivales. Por otro lado, se ha demostrado que la acumulación del biofilm y la inflamación gingival aumentan la prevalencia de bacteriemias tras manipulaciones bucodentales profesionales y no profesionales, lo que podría justificar el análisis de esas bacterias a nivel subgingival en estos pacientes. OBJETIVOS: Validar y comparar los resultados obtenidos con diferentes técnicas microbiológicas para la detección de patógenos clave en muestras de sangre y en muestras subgingivales para el estudio de bacteriemias de origen periodontal. CONCLUSIONES: Los resultados de esta serie de estudios demuestran que la detección de patógenos periodontales, tanto en muestras de sangre como en muestras subgingivales, requiere de procesamiento y técnicas validadas para su identificación y cuantificación. Parece que las técnicas utilizadas en microbiología periodontal, tales como el CAD o la qPCR son las técnicas de elección en aquellos estudios en los que se quiera evaluar el paso de patógenos periodontales al torrente sanguíneo...
BACKGROUND: The entrance of periodontal pathogens ino the blood circulation (bacteriemia) has been postulated as one of the potential mechanisms responsible for the relationship between periodontitis and certain systemic diseases. In addition, not many studies have analyzed and correlated the data obtained in blood samples with those obtained in subgingival samples. Furthermore, biofilm accumulation and gingival inflammation have been shown to increase the prevalence of bacteriemia after professional and non-professional oral manipulations, which could justify the analysis of these periodontal pathogens in patients suffering from bacteremia. AIM: To validate and compare the results obtained with different microbiological techniques for the detection of periodontal pathogens in blood samples and in subgingival samples for the evaluation of bacteremia of periodontal origin.CONCLUSIONS: The results of these series of studies show that the detection of periodontal bacteria both in blood samples and in subgingival samples requires adequate and validated techniquesfor their identification and quantification. It seems that the techniques used in periodontal microbiology, such as culture or qPCR, are the techniques of choice when trying to assess the entrance of periodontal pathogens into the bloodstream...
BACKGROUND: The entrance of periodontal pathogens ino the blood circulation (bacteriemia) has been postulated as one of the potential mechanisms responsible for the relationship between periodontitis and certain systemic diseases. In addition, not many studies have analyzed and correlated the data obtained in blood samples with those obtained in subgingival samples. Furthermore, biofilm accumulation and gingival inflammation have been shown to increase the prevalence of bacteriemia after professional and non-professional oral manipulations, which could justify the analysis of these periodontal pathogens in patients suffering from bacteremia. AIM: To validate and compare the results obtained with different microbiological techniques for the detection of periodontal pathogens in blood samples and in subgingival samples for the evaluation of bacteremia of periodontal origin.CONCLUSIONS: The results of these series of studies show that the detection of periodontal bacteria both in blood samples and in subgingival samples requires adequate and validated techniquesfor their identification and quantification. It seems that the techniques used in periodontal microbiology, such as culture or qPCR, are the techniques of choice when trying to assess the entrance of periodontal pathogens into the bloodstream...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Odontología, leída el 27-01-2020