Publication: Alteraciones asociadas al metabolismo de los aneurismas de aorta abdominal: estudio proteómico
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2017-08-21
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Universidad Complutense de Madrid
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Abstract
Los aneurismas de aorta abdominal (AAA), con una prevalencia de hasta el 9% en varones mayores de 65 años, se caracterizan por la dilatación de la pared aórtica de forma opuesta a la manifestación oclusiva de la ateroesclerosis, con una mortalidad d e hasta el 80% en casos de rotura. En las últimas décadas, se han publicado numerosos estudios en relación a la etiopatogenia de entidad, aún en discusión. No obstante, la mayoría de ellos abogan por la inflamación de la pared aórtica y destrucción estructural de la misma como una de las características principales de los mismos. Sin embargo, escasas son las referencias al metabolismo energético celular que permite la consecución de todas los procesos de señalización, transporte y regulación de expresión génica que resultan en dichos cambios estructurales. Desde el punto de vista analítico, la identificación masiva de las proteínas de una misma muestra es actualmente factible gracias a la tecnología englobada en la proteómica, que incluye el análisis por electroforesis bidimensional y la espectrometría de masas. Esta aproximación metodológica, aplicada a la salud, permite analizar, cuantificar e identificar los mapas proteicos de una misma muestra y su comparación con otras no afectas de enfermedad...
Abdominal aortic aneurysms (AAA) occur in up to 9% of males over 65 years of age, and are characterized by aortic dilation opposed to the characteristic occlusion of atherosclerotic disease. Mortality rates in case of rupture exceed 80%. Over the last decades, multiple studies have been published regarding AAA pathogenesis, still unclear. Different findings have demonstrated that both degradation of the extracellular matrix accompanied by chronic inflammation are characteristic processes associated to aneurysm formation. However, data regarding energetic metabolism responsible for all signaling reactions, biomolecule transportation and gene expression regulation are scarce. Molecular technologies involved in proteomics, including two-dimensional electrophoresis and mass spectrometry, have become widely available and allow massive analysis of complex protein mixtures. Thus, proteomics allow us to analyze, quantify and identify a single sample protein expression pattern and its comparison to others. The aim of the present study was then to analyze the expression of glucose metabolism-related proteins at the aneurysmal site compared to healthy aorta using proteomics, to analyze enzyme metabolic activity, content of both pyruvate and lactate, and to determine the expression of cellular glucose transporter GLUT-3...
Abdominal aortic aneurysms (AAA) occur in up to 9% of males over 65 years of age, and are characterized by aortic dilation opposed to the characteristic occlusion of atherosclerotic disease. Mortality rates in case of rupture exceed 80%. Over the last decades, multiple studies have been published regarding AAA pathogenesis, still unclear. Different findings have demonstrated that both degradation of the extracellular matrix accompanied by chronic inflammation are characteristic processes associated to aneurysm formation. However, data regarding energetic metabolism responsible for all signaling reactions, biomolecule transportation and gene expression regulation are scarce. Molecular technologies involved in proteomics, including two-dimensional electrophoresis and mass spectrometry, have become widely available and allow massive analysis of complex protein mixtures. Thus, proteomics allow us to analyze, quantify and identify a single sample protein expression pattern and its comparison to others. The aim of the present study was then to analyze the expression of glucose metabolism-related proteins at the aneurysmal site compared to healthy aorta using proteomics, to analyze enzyme metabolic activity, content of both pyruvate and lactate, and to determine the expression of cellular glucose transporter GLUT-3...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Medicina, Departamento de Cirugía, leída el 21-01-2016