Publication: Dinámica del quimerismo en sangre periférica, médula ósea y linajes leucocitarios en el trasplante alogénico de progenitores hematopoyéticos
Loading...
Files
Official URL
Full text at PDC
Publication Date
2020-01-13
Authors
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Universidad Complutense de Madrid
Citations
Exportar
Abstract
El trasplante alogénico de progenitores hematopoyéticos (alo-TPH) se basa en la sustitución completa de una hematopoyesis enferma por la de un donante sano, y a día de hoy constituye la única opción curativa en múltiples patologías. No obstante, es un tratamiento altamente complejo que requiere un control muy estrecho de los pacientes. La cuantificación de ADN de donante y de receptor, o análisis del quimerismo, constituye una herramienta para monitorizar el éxito del procedimiento y es crucial en el diagnóstico y manejo de algunas complicaciones. El objetivo de este trabajo consistió en analizar y comparar la dinámica del quimerismo en sangre periférica (SP), médula ósea (MO) y linajes leucocitarios y estudiar su relación con factores pretrasplante y con el desarrollo de complicaciones. Se analizó el quimerismo en 199 pacientes que recibieron alo-TPH en el Hospital General Universitario Gregorio Marañón entre los años 2010 y 2015. El análisis se realizó mediante PCR de microsatélites en SP y MO totales y en diferentes linajes leucocitarios separados mediante tecnología inmunomagnética...
Allogeneic hematopoietic stem cell transplant (allo-HSCT) consists in the complete replacement of a damaged haematopoiesis by another from a healthy donor. It is nowadays the only curative option for different diseases. However, it is a highly complex treatment, which requires strict monitoring of patients. Chimerism analysis (donor and recipient DNA quantification), is a useful tool in assessing adequate evolution of the procedure and it is crucial in diagnosing and managing several complications. Our objective was to analyse and compare chimerism dynamics in peripheral blood (PB), bone marrow (BM) and leukocyte linages as well as to study the relationship between chimerism and several pre-transplant factors and the development of complications. We included 199 allo-HSCT recipients transplanted at Hospital General Universitario Gregorio Marañón between 2010 and 2015. Chimerism analysis was performed using microsatellite PCR in PB, BM and different leukocyte subsets, obtained by immunomagnetic separation...
Allogeneic hematopoietic stem cell transplant (allo-HSCT) consists in the complete replacement of a damaged haematopoiesis by another from a healthy donor. It is nowadays the only curative option for different diseases. However, it is a highly complex treatment, which requires strict monitoring of patients. Chimerism analysis (donor and recipient DNA quantification), is a useful tool in assessing adequate evolution of the procedure and it is crucial in diagnosing and managing several complications. Our objective was to analyse and compare chimerism dynamics in peripheral blood (PB), bone marrow (BM) and leukocyte linages as well as to study the relationship between chimerism and several pre-transplant factors and the development of complications. We included 199 allo-HSCT recipients transplanted at Hospital General Universitario Gregorio Marañón between 2010 and 2015. Chimerism analysis was performed using microsatellite PCR in PB, BM and different leukocyte subsets, obtained by immunomagnetic separation...
Description
Tesis inédita de la Universidad Complutense de Madrid, Facultad de Medicina, Departamento de Medicina, leída el 19-11-2019