Publication: Alteraciones oculares embrionarias por déficit dietario materno de ácido fólico: análisis de imagen, caracterización óptica y biológica
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2020-10-28
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Universidad Complutense de Madrid
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Abstract
La presente tesis doctoral que lleva por título “Alteraciones oculares embrionarias por déficit dietario materno de ácido fólico: análisis de imagen, caracterización óptica y biológica” aborda el estudio óptico y biológico de tejidos oculares de embriones de ratón cuyas madres fueron sometidas a una dieta carente en ácido fólico (AF). Los objetivos generales y específicos del estudio eran: identificar el tipo de malformaciones oculares congénitas producidas a causa del déficit materno de AF y realizar un estudio estadístico para conocer su frecuencia en los embriones, estudiar cambios producidos en la biometría de algunas estructuras oculares aplicando la técnica estadística de remuestreo de datos Bootstrap, determinar algunos mecanismos alterados implementando una metodología automatizada para cuantificar y regionalizar la expresión de dos moléculas de la matriz extracelular, colágeno IV y laminina-1, en el interior de algunas estructuras del ojo, utilizar las técnicas de procesado y de análisis de imagen para analizar los cambios producidos en la textura de los tejidos, utilizar la herramienta de análisis multifractal para, evaluar, cuantificar y compara los cambios en la textura de los tejidos. Finalmente, averiguar cambios en el comportamiento espectral de los tejidos mediante dos técnicas ópticas: polarización de la luz e imágenes multiespectrales...
The present doctoral thesis entitled "Embryonic eye disorders due to maternal dietary deficiency of folic acid: image analysis, optical and biological characterization" addresses the optical and biological study of ocular tissues of mouse embryos whose mothers were subjected to a diet lacking in folic acid (FA). The general and specific objectives of the study were: identify the type of congenital eye malformations produced due to the maternal FA deficiency and perform a statistical study to know their frequency in embryos, to study changes in the biometry of some ocular structures by applying the bootstrap resampling technique, determine some altered mechanisms by implementing a methodology to quantify and regionalize the expression of two molecules of the extracellular matrix, collagen IV and laminin-1, inside some structures of the eye, use the techniques of image processing and analysis to analyze the changes produced in the texture of the tissues, use the multifractal analysis tool to evaluate, quantify and compare changes in the texture of tissues. Finally, find out changes in the spectral behavior of the tissues by means of two optical techniques: polarization of light and multispectral images...
The present doctoral thesis entitled "Embryonic eye disorders due to maternal dietary deficiency of folic acid: image analysis, optical and biological characterization" addresses the optical and biological study of ocular tissues of mouse embryos whose mothers were subjected to a diet lacking in folic acid (FA). The general and specific objectives of the study were: identify the type of congenital eye malformations produced due to the maternal FA deficiency and perform a statistical study to know their frequency in embryos, to study changes in the biometry of some ocular structures by applying the bootstrap resampling technique, determine some altered mechanisms by implementing a methodology to quantify and regionalize the expression of two molecules of the extracellular matrix, collagen IV and laminin-1, inside some structures of the eye, use the techniques of image processing and analysis to analyze the changes produced in the texture of the tissues, use the multifractal analysis tool to evaluate, quantify and compare changes in the texture of tissues. Finally, find out changes in the spectral behavior of the tissues by means of two optical techniques: polarization of light and multispectral images...
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Tesis inédita de la Universidad Complutense de Madrid, Facultad de Óptica, leída el 21-11-2019